Gene description for Hspa8 |
Gene name |
heat shock 70kDa protein 8 |
Gene symbol |
Hspa8 |
Other names/aliases |
Hsc70 |
Species |
Rattus norvegicus |
Database cross references - Hspa8 |
Vesiclepedia |
VP_24468 |
ExoCarta |
ExoCarta_24468 |
Entrez Gene |
24468 |
Hspa8 identified in extracellular vesicles derived from the following tissue/cell type |
Cardiomyocytes [Exosomes] More >>>
|
23376832
|
Cortical neurones [Exosomes] More >>>
|
16446100
|
Hepatocytes [Exosomes] More >>>
|
19367702
|
Insulinoma cells [Exosomes] More >>>
|
27872147
|
Insulinoma cells [Exosomes] More >>>
|
27872147
|
Insulinoma cells [Microparticles] More >>>
|
22345510
|
Insulinoma cells [Microparticles] More >>>
|
22345510
|
Insulinoma cells [Microparticles] More >>>
|
22345510
|
Insulinoma cells [Exosomes] More >>>
|
22345510
|
Insulinoma cells [Exosomes] More >>>
|
22345510
|
Insulinoma cells [Exosomes] More >>>
|
22345510
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Reticulocytes [Exosomes] More >>>
|
21828046
|
Retinal glial cells [Exosomes] More >>>
|
24400796
|
Experiment description of studies that identified Hspa8 in extracellular vesicles |
1
|
Experiment ID | 831 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
23376832
|
Organism | Rattus norvegicus |
Experiment description | Cardiac myocyte exosomes: stability, HSP60, and proteomics |
Authors | Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. |
Journal name |
Am J Physiol Heart Circ Physiol
|
Publication year | 2013 |
Sample | Cardiomyocytes |
Sample name | Cardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 1 |
Isolation/purification methods | Differential centrifugation Filtration ExoQuick Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV130100: EV-METRIC:38%, 13%
|
|
|
2
|
Experiment ID | 30 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
16446100
|
Organism | Rattus norvegicus |
Experiment description | Exosomes are released by cultured cortical neurones. |
Authors | Fauré, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R |
Journal name |
MCN
|
Publication year | 2006 |
Sample | Cortical neurones |
Sample name | Neurones |
Isolation/purification methods | Differential centrifugation Sucrose density gradient |
Flotation density | 1.13 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [QTOF] Western blotting |
EV-TRACK |
-
|
|
|
3
|
Experiment ID | 35 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
19367702
|
Organism | Rattus norvegicus |
Experiment description | Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. |
Authors | Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM |
Journal name |
JPR
|
Publication year | 2008 |
Sample | Hepatocytes |
Sample name | Hepatocytes |
Isolation/purification methods | Differential centrifugation Filtration Sucrose density gradient |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [QTOF] Western blotting |
EV-TRACK |
-
|
|
|
4
|
Experiment ID | 1220 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
27872147
|
Organism | Rattus norvegicus |
Experiment description | Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. |
Authors | Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. |
Journal name |
Diabetes.
|
Publication year | 2017 |
Sample | Insulinoma cells |
Sample name | INS-1E cells |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160008: EV-METRIC:55%, 44%, 33%
|
|
|
5
|
Experiment ID | 1221 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
27872147
|
Organism | Rattus norvegicus |
Experiment description | Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. |
Authors | Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. |
Journal name |
Diabetes.
|
Publication year | 2017 |
Sample | Insulinoma cells |
Sample name | Rat interleukin (IL)-1. (10 units/mL) |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160008: EV-METRIC:55%, 44%, 33%
|
|
|
6
|
Experiment ID | 1279 |
Identified molecule | protein
|
Extracellular vesicle type | Microparticles |
Identification method | Mass spectrometry
|
PubMed ID |
22345510
|
Organism | Rattus norvegicus |
Experiment description | Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. |
Authors | Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. |
Journal name |
Mol Cell Proteomics.
|
Publication year | 2012 |
Sample | Insulinoma cells |
Sample name | NHI 6F Tu28, cytokine-stimulated Replicate 1 |
Isolation/purification methods | Differential centrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV120151: EV-METRIC:14%, 0%
|
|
|
7
|
Experiment ID | 1280 |
Identified molecule | protein
|
Extracellular vesicle type | Microparticles |
Identification method | Mass spectrometry
|
PubMed ID |
22345510
|
Organism | Rattus norvegicus |
Experiment description | Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. |
Authors | Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. |
Journal name |
Mol Cell Proteomics.
|
Publication year | 2012 |
Sample | Insulinoma cells |
Sample name | NHI 6F Tu28, cytokine-stimulated Replicate 2 |
Isolation/purification methods | Differential centrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV120151: EV-METRIC:14%, 0%
|
|
|
8
|
Experiment ID | 1281 |
Identified molecule | protein
|
Extracellular vesicle type | Microparticles |
Identification method | Mass spectrometry
|
PubMed ID |
22345510
|
Organism | Rattus norvegicus |
Experiment description | Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. |
Authors | Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. |
Journal name |
Mol Cell Proteomics.
|
Publication year | 2012 |
Sample | Insulinoma cells |
Sample name | NHI 6F Tu28, cytokine-stimulated Replicate 3 |
Isolation/purification methods | Differential centrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV120151: EV-METRIC:14%, 0%
|
|
|
9
|
Experiment ID | 1282 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
22345510
|
Organism | Rattus norvegicus |
Experiment description | Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. |
Authors | Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. |
Journal name |
Mol Cell Proteomics.
|
Publication year | 2012 |
Sample | Insulinoma cells |
Sample name | NHI 6F Tu28, cytokine-stimulated Replicate 1 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV120151: EV-METRIC:14%, 0%
|
|
|
10
|
Experiment ID | 1283 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
22345510
|
Organism | Rattus norvegicus |
Experiment description | Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. |
Authors | Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. |
Journal name |
Mol Cell Proteomics.
|
Publication year | 2012 |
Sample | Insulinoma cells |
Sample name | NHI 6F Tu28, cytokine-stimulated Replicate 2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV120151: EV-METRIC:14%, 0%
|
|
|
11
|
Experiment ID | 1284 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
22345510
|
Organism | Rattus norvegicus |
Experiment description | Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. |
Authors | Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. |
Journal name |
Mol Cell Proteomics.
|
Publication year | 2012 |
Sample | Insulinoma cells |
Sample name | NHI 6F Tu28, cytokine-stimulated Replicate 3 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV120151: EV-METRIC:14%, 0%
|
|
|
12
|
Experiment ID | 90 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D2 Rep 1 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.16-1.21 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
13
|
Experiment ID | 94 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D4 Rep 1 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.16-1.21 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
14
|
Experiment ID | 95 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D7 Rep 1 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.13-1.25 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
15
|
Experiment ID | 96 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D2 Rep 2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.16-1.21 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
16
|
Experiment ID | 97 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D4 Rep 2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.16-1.21 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
17
|
Experiment ID | 98 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D7 Rep 2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.13-1.25 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
18
|
Experiment ID | 99 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [QSTAR]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D2 Rep 3 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.16-1.21 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
19
|
Experiment ID | 100 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [QSTAR]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D4 Rep 3 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.16-1.21 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
20
|
Experiment ID | 101 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry [QSTAR]
|
PubMed ID |
21828046
|
Organism | Rattus norvegicus |
Experiment description | The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors | Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. |
Journal name |
JBC
|
Publication year | 2011 |
Sample | Reticulocytes |
Sample name | Reticulocytes - D7 Rep 3 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.13-1.25 g/mL |
Molecules identified in the study | Protein Lipids |
Methods used in the study | Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
EV-TRACK |
EV110005: EV-METRIC:44%
|
|
|
21
|
Experiment ID | 1259 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
Identification method | Mass spectrometry
|
PubMed ID |
24400796
|
Organism | Rattus norvegicus |
Experiment description | Exosomal proteins in the aqueous humor as novel biomarkers in patients with neovascular age-related macular degeneration. |
Authors | Kang GY, Bang JY, Choi AJ, Yoon J, Lee WC, Choi S, Yoon S, Kim HC, Baek JH, Park HS, Lim HJ, Chung H. |
Journal name |
J Proteome Res.
|
Publication year | 2014 |
Sample | Retinal glial cells |
Sample name | Mücells |
Isolation/purification methods | ExoQuick |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV140174: EV-METRIC:13%, 13%
|
|
|