Gene description for Hspa8
Gene name	heat shock 70kDa protein 8
Gene symbol	Hspa8
Other names/aliases	Hsc70
Species	Rattus norvegicus
Database cross references - Hspa8
Vesiclepedia	VP_24468
ExoCarta	ExoCarta_24468
Entrez Gene	24468
Hspa8 identified in extracellular vesicles derived from the following tissue/cell type
Cardiomyocytes [Exosomes]      More >>>	23376832
Cortical neurones [Exosomes]      More >>>	16446100
Hepatocytes [Exosomes]      More >>>	19367702
Insulinoma cells [Exosomes]      More >>>	27872147
Insulinoma cells [Exosomes]      More >>>	27872147
Insulinoma cells [Microparticles]      More >>>	22345510
Insulinoma cells [Microparticles]      More >>>	22345510
Insulinoma cells [Microparticles]      More >>>	22345510
Insulinoma cells [Exosomes]      More >>>	22345510
Insulinoma cells [Exosomes]      More >>>	22345510
Insulinoma cells [Exosomes]      More >>>	22345510
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Reticulocytes [Exosomes]      More >>>	21828046
Retinal glial cells [Exosomes]      More >>>	24400796
Experiment description of studies that identified Hspa8 in extracellular vesicles
1 Experiment ID 831 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 1 Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
2 Experiment ID 30 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 16446100    Organism Rattus norvegicus Experiment description Exosomes are released by cultured cortical neurones. Authors Fauré, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R Journal name MCN Publication year 2006 Sample Cortical neurones Sample name Neurones Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.13 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting EV-TRACK -
3 Experiment ID 35 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 19367702    Organism Rattus norvegicus Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM Journal name JPR Publication year 2008 Sample Hepatocytes Sample name Hepatocytes Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting EV-TRACK -
4 Experiment ID 1220 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name INS-1E cells Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
5 Experiment ID 1221 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name Rat interleukin (IL)-1. (10 units/mL) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
6 Experiment ID 1279 Identified molecule protein Extracellular vesicle type Microparticles Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 1 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
7 Experiment ID 1280 Identified molecule protein Extracellular vesicle type Microparticles Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 2 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
8 Experiment ID 1281 Identified molecule protein Extracellular vesicle type Microparticles Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
9 Experiment ID 1282 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
10 Experiment ID 1283 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
11 Experiment ID 1284 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
12 Experiment ID 90 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
13 Experiment ID 94 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
14 Experiment ID 95 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
15 Experiment ID 96 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
16 Experiment ID 97 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
17 Experiment ID 98 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
18 Experiment ID 99 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [QSTAR] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
19 Experiment ID 100 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [QSTAR] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
20 Experiment ID 101 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [QSTAR] PubMed ID 21828046    Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting EV-TRACK EV110005: EV-METRIC:44%
21 Experiment ID 1259 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 24400796    Organism Rattus norvegicus Experiment description Exosomal proteins in the aqueous humor as novel biomarkers in patients with neovascular age-related macular degeneration. Authors Kang GY, Bang JY, Choi AJ, Yoon J, Lee WC, Choi S, Yoon S, Kim HC, Baek JH, Park HS, Lim HJ, Chung H. Journal name J Proteome Res. Publication year 2014 Sample Retinal glial cells Sample name Mücells Isolation/purification methods ExoQuick Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140174: EV-METRIC:13%, 13%