Gene description for Anxa5
Gene name annexin A5
Gene symbol Anxa5
Other names/aliases Anx5
LC5
Species Rattus norvegicus
 Database cross references - Anxa5
Vesiclepedia VP_25673
ExoCarta ExoCarta_25673
Entrez Gene 25673
 Anxa5 identified in extracellular vesicles derived from the following tissue/cell type
Hepatocytes [Exosomes]      More >>> 19367702   
Primary microglial cells [Exosomes]      More >>> 23173708   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Urine [Exosomes]      More >>> 20535238   
 Experiment description of studies that identified Anxa5 in extracellular vesicles
1
Experiment ID35
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19367702   
OrganismRattus norvegicus
Experiment descriptionCharacterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year2008
SampleHepatocytes
Sample nameHepatocytes
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
2
Experiment ID1145
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (8 hour)
100,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120065: EV-METRIC:22%, 22%
3
Experiment ID90
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
4
Experiment ID94
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
5
Experiment ID95
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
6
Experiment ID97
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
7
Experiment ID98
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
8
Experiment ID413
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20535238   
OrganismRattus norvegicus
Experiment descriptionCandidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year2010
SampleUrine
Sample nameRats of liver injury-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV100012: EV-METRIC:33%, 33%