Gene description for Tsg101
Gene name tumor susceptibility gene 101
Gene symbol Tsg101
Other names/aliases Rw
Species Rattus norvegicus
 Database cross references - Tsg101
Vesiclepedia VP_292925
ExoCarta ExoCarta_292925
Entrez Gene 292925
 Tsg101 identified in extracellular vesicles derived from the following tissue/cell type
Cortical neurones [Exosomes]      More >>> 16446100   
Hepatocytes [Exosomes]      More >>> 19367702   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Urine [Exosomes]      More >>> 23344851   
Urine [Exosomes]      More >>> 23344851   
 Experiment description of studies that identified Tsg101 in extracellular vesicles
1
Experiment ID30
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 16446100   
OrganismRattus norvegicus
Experiment descriptionExosomes are released by cultured cortical neurones.
AuthorsFauré, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year2006
SampleCortical neurones
Sample nameNeurones
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
2
Experiment ID35
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 19367702   
OrganismRattus norvegicus
Experiment descriptionCharacterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year2008
SampleHepatocytes
Sample nameHepatocytes
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
3
Experiment ID96
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
Western blotting
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
4
Experiment ID97
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
Western blotting
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
5
Experiment ID98
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
6
Experiment ID1276
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23344851   
OrganismRattus norvegicus
Experiment descriptionUrinary exosomes and diabetic nephropathy: a proteomic approach.
AuthorsRaimondo F, Corbetta S, Morosi L, Chinello C, Gianazza E, Castoldi G, Di Gioia C, Bombardi C, Stella A, Battaglia C, Bianchi C, Magni F, Pitto M.
Journal name Mol Biosyst.
Publication year2013
SampleUrine
Sample nameControl
Isolation/purification methodsUltracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130052: EV-METRIC:22%
7
Experiment ID1277
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23344851   
OrganismRattus norvegicus
Experiment descriptionUrinary exosomes and diabetic nephropathy: a proteomic approach.
AuthorsRaimondo F, Corbetta S, Morosi L, Chinello C, Gianazza E, Castoldi G, Di Gioia C, Bombardi C, Stella A, Battaglia C, Bianchi C, Magni F, Pitto M.
Journal name Mol Biosyst.
Publication year2013
SampleUrine
Sample nameDiabetic
Isolation/purification methodsUltracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130052: EV-METRIC:22%