Gene description for Gsn
Gene name gelsolin
Gene symbol Gsn
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gsn
Vesiclepedia VP_296654
ExoCarta ExoCarta_296654
Entrez Gene 296654
 Gsn identified in extracellular vesicles derived from the following tissue/cell type
Cortical neurones [Exosomes]      More >>> 16446100   
Pancreatic adenocarcinoma cells [Exosomes]      More >>> 20124479   
Pancreatic adenocarcinoma cells [Exosomes]      More >>> 20124479   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Retinal glial cells [Exosomes]      More >>> 24400796   
 Experiment description of studies that identified Gsn in extracellular vesicles
1
Experiment ID30
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 16446100   
OrganismRattus norvegicus
Experiment descriptionExosomes are released by cultured cortical neurones.
AuthorsFauré, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year2006
SampleCortical neurones
Sample nameNeurones
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
2
Experiment ID92
Identified moleculemrna
Extracellular vesicle typeExosomes
Identification method Microarray
PubMed ID 20124479   
OrganismRattus norvegicus
Experiment descriptionCell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
AuthorsNazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zör M.
Journal name CR
Publication year2010
SamplePancreatic adenocarcinoma cells
Sample nameAS
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14-1.17 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
EV-TRACK EV100025: EV-METRIC:44%, 44%
3
Experiment ID93
Identified moleculemrna
Extracellular vesicle typeExosomes
Identification method Microarray
PubMed ID 20124479   
OrganismRattus norvegicus
Experiment descriptionCell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
AuthorsNazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zör M.
Journal name CR
Publication year2010
SamplePancreatic adenocarcinoma cells
Sample nameAS-Tspan8
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14-1.17 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-PCR
Western blotting
EV-TRACK EV100025: EV-METRIC:44%, 44%
4
Experiment ID94
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
5
Experiment ID95
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
6
Experiment ID96
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
7
Experiment ID97
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
8
Experiment ID98
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
9
Experiment ID1259
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24400796   
OrganismRattus norvegicus
Experiment descriptionExosomal proteins in the aqueous humor as novel biomarkers in patients with neovascular age-related macular degeneration.
AuthorsKang GY, Bang JY, Choi AJ, Yoon J, Lee WC, Choi S, Yoon S, Kim HC, Baek JH, Park HS, Lim HJ, Chung H.
Journal name J Proteome Res.
Publication year2014
SampleRetinal glial cells
Sample nameMücells
Isolation/purification methodsExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140174: EV-METRIC:13%, 13%