Gene description for HLA-DRB5
Gene name major histocompatibility complex, class II, DR beta 5
Gene symbol HLA-DRB5
Other names/aliases HLA-DRB
Species Homo sapiens
 Database cross references - HLA-DRB5
Vesiclepedia VP_3127
ExoCarta ExoCarta_3127
Entrez Gene 3127
HGNC 4953
MIM 604776
 HLA-DRB5 identified in extracellular vesicles derived from the following tissue/cell type
Ascites [Microvesicles]      More >>> 21630462   
B cells [Exosomes]      More >>> 12519789   
B cells [Exosomes]      More >>> 9685355   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 10903724   
B cells [Exosomes]      More >>> 20304794   
Breast milk [Exosomes]      More >>> 21235781   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Burkitt's lymphoma [Microvesicles]      More >>> 9126269   
Burkitt's lymphoma [Microvesicles]      More >>> 9126269   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Dendritic cells [Microvesicles]      More >>> 10903724   
Dendritic cells [Exosomes]      More >>> 26858453   
Glioblastoma cells [Microvesicles]      More >>> 19011622   
Malignant pleural effusions [Exosomes/Microvesicles/Ectosomes/Microparticles]      More >>> 23585444   
Plasma [Exosomes]      More >>> 21235781   
Saliva [Exosomes]      More >>> 21235781   
T cells [Exosomes]      More >>> 11907077   
T cells [Microvesicles]      More >>> 9126269   
T cells [Microvesicles]      More >>> 9126269   
T cells [Exosomes]      More >>> 23463506   
Thymus [Exosomes]      More >>> 23844026   
 Experiment description of studies that identified HLA-DRB5 in extracellular vesicles
1
Experiment ID159
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%
2
Experiment ID11
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [MALDI TOF]
Western blotting
PubMed ID 12519789   
OrganismHomo sapiens
Experiment descriptionProteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
AuthorsWubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mös W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name JBC
Publication year2003
SampleB cells
Sample nameRN (HLA-DR15+)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.15 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
High performance liquid chromatography
EV-TRACK -
3
Experiment ID16
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 9685355   
OrganismHomo sapiens
Experiment descriptionSelective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
AuthorsEscola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name JBC
Publication year1998
SampleB cells
Sample nameRN (HLA-DR15+)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Immunoelectron microscopy
EV-TRACK -
4
Experiment ID79
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
5
Experiment ID80
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
6
Experiment ID81
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
7
Experiment ID271
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Immunoelectron microscopy
PubMed ID 10903724   
OrganismHomo sapiens
Experiment descriptionFollicular dendritic cells carry MHC class II-expressing microvesicles at their surface
AuthorsDenzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ
Journal name J Immunol
Publication year2000
SampleB cells
Sample nameRN
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImmunoelectron microscopy
EV-TRACK -
8
Experiment ID323
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 20304794   
OrganismHomo sapiens
Experiment descriptionFunctional delivery of viral miRNAs via exosomes
AuthorsPegtel DM, Cosmopoulos K, Thorley-Lawson DA, van Eijndhoven MA, Hopmans ES, Lindenberg JL, de Gruijl TD, Wüer T, Middeldorp JM
Journal name Proc Natl Acad Sci U S A
Publication year2010
SampleB cells
Sample nameB cells - Transformed with Epstein–Barr virus
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV100051: EV-METRIC:22%
9
Experiment ID124
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method FACS
PubMed ID 21235781   
OrganismHomo sapiens
Experiment descriptionHuman saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
AuthorsLäer C, Alikhani VS, Ekströ, Eldh M, Paredes PT, Bossios A, Sjöand M, Gabrielsson S, Löll J, Valadi H.
Journal name JTM
Publication year2011
SampleBreast milk
Sample nameBreast milk - normal
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Immunoelectron microscopy
Western blotting
EV-TRACK EV110029: EV-METRIC:14%, 29%, 14%
10
Experiment ID574
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive]
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components
AuthorsVan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name MCP
Publication year2016
SampleBreast milk
Sample nameBreast milk - normal
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
11
Experiment ID952
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
12
Experiment ID953
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%
13
Experiment ID954
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D5
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%
14
Experiment ID956
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D7
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%
15
Experiment ID957
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - pooled
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
16
Experiment ID255
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Radioimmunoassay
PubMed ID 9126269   
OrganismHomo sapiens
Experiment descriptionMicrovesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations
AuthorsBess JW Jr, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO
Journal name Virology
Publication year1997
SampleBurkitt's lymphoma
Sample nameDAUDI - HIV non infected
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.08-1.29 g/mL
Molecules identified in the studyProtein
Methods used in the studyRadioimmunoassay
EV-TRACK -
17
Experiment ID256
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Radioimmunoassay
PubMed ID 9126269   
OrganismHomo sapiens
Experiment descriptionMicrovesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations
AuthorsBess JW Jr, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO
Journal name Virology
Publication year1997
SampleBurkitt's lymphoma
Sample nameDAUDI - HIV infected
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.08-1.29 g/mL
Molecules identified in the studyProtein
Methods used in the studyRadioimmunoassay
EV-TRACK -
18
Experiment ID899
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameCLL cells - Rep 1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV150015: EV-METRIC:22%, 44%
19
Experiment ID900
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameCLL cells - Rep 2
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV150015: EV-METRIC:22%, 44%
20
Experiment ID901
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameJVM-3
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV150015: EV-METRIC:22%, 44%
21
Experiment ID902
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameMEC-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
FACS
Western blotting
EV-TRACK EV150015: EV-METRIC:22%, 44%
22
Experiment ID270
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Immunoelectron microscopy
PubMed ID 10903724   
OrganismHomo sapiens
Experiment descriptionFollicular dendritic cells carry MHC class II-expressing microvesicles at their surface
AuthorsDenzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ
Journal name J Immunol
Publication year2000
SampleDendritic cells
Sample nameFollicular dendritic cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImmunoelectron microscopy
EV-TRACK -
23
Experiment ID564
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
24
Experiment ID203
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray
PubMed ID 19011622   
OrganismHomo sapiens
Experiment descriptionGlioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
AuthorsSkog J, Wüer T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO
Journal name Nat Cell Biol
Publication year2008
SampleGlioblastoma cells
Sample nameGlioblastoma cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
miRNA
mRNA
Methods used in the studyRT-PCR
Antibody array
Microarray
EV-TRACK -
25
Experiment ID519
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Ectosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23585444   
OrganismHomo sapiens
Experiment descriptionIdentification and characterization of proteins isolated from microvesicles derived from human lung cancer pleural effusions.
AuthorsPark JO, Choi DY, Choi DS, Kim HJ, Kang JW, Jung JH, Lee JH, Kim J, Freeman MR, Lee KY, Gho YS, Kim KP.
Journal name Proteomics
Publication year2013
SampleMalignant pleural effusions
Sample nameNon-small cell lung cancer patient-Lung cancer pleural effusion
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130049: EV-METRIC:38%
26
Experiment ID123
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method FACS
PubMed ID 21235781   
OrganismHomo sapiens
Experiment descriptionHuman saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
AuthorsLäer C, Alikhani VS, Ekströ, Eldh M, Paredes PT, Bossios A, Sjöand M, Gabrielsson S, Löll J, Valadi H.
Journal name JTM
Publication year2011
SamplePlasma
Sample namePlasma - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Immunoelectron microscopy
Western blotting
EV-TRACK EV110029: EV-METRIC:14%, 29%, 14%
27
Experiment ID122
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method FACS
PubMed ID 21235781   
OrganismHomo sapiens
Experiment descriptionHuman saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
AuthorsLäer C, Alikhani VS, Ekströ, Eldh M, Paredes PT, Bossios A, Sjöand M, Gabrielsson S, Löll J, Valadi H.
Journal name JTM
Publication year2011
SampleSaliva
Sample nameSaliva - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Immunoelectron microscopy
Western blotting
EV-TRACK EV110029: EV-METRIC:14%, 29%, 14%
28
Experiment ID18
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method FACS
PubMed ID 11907077   
OrganismHomo sapiens
Experiment descriptionTCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
AuthorsBlanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name JIMMU
Publication year2002
SampleT cells
Sample nameJurkat
T cell blasts
Isolation/purification methodsFiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK -
29
Experiment ID245
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Radioimmunoassay
PubMed ID 9126269   
OrganismHomo sapiens
Experiment descriptionMicrovesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations
AuthorsBess JW Jr, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO
Journal name Virology
Publication year1997
SampleT cells
Sample nameH9 - non infected
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.08-1.29 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyRadioimmunoassay
EV-TRACK -
30
Experiment ID252
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Radioimmunoassay
PubMed ID 9126269   
OrganismHomo sapiens
Experiment descriptionMicrovesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations
AuthorsBess JW Jr, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO
Journal name Virology
Publication year1997
SampleT cells
Sample nameH9 - HIV infected
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.08-1.29 g/mL
Molecules identified in the studyProtein
Methods used in the studyRadioimmunoassay
Northern blotting
EV-TRACK -
31
Experiment ID1008
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismHomo sapiens
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130110: EV-METRIC:22%
32
Experiment ID538
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23844026   
OrganismHomo sapiens
Experiment descriptionCharacterization of human thymic exosomes.
AuthorsSkogberg G, Gudmundsdottir J, van der Post S, Sandström K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year2013
SampleThymus
Sample nameNormal-Thymus
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130018: EV-METRIC:63%