Gene description for Rala
Gene name	Ras-related protein
Gene symbol	Rala
Other names/aliases	CG2849 D-RalA DRal DRala Dm RalA DmelCG2849 Dral DralA Drala RALA Ral Ral-a RalA anon-EST:fe1H4 dRal l(1)G0174 l(1)G0373 l(1)G0501 ral ralA
Species	Drosophila melanogaster
Database cross references - Rala
Vesiclepedia	VP_31332
ExoCarta	ExoCarta_31332
Entrez Gene	31332
Rala identified in extracellular vesicles derived from the following tissue/cell type
Drosophila S2 cells [Exosomes]      More >>>	23035643
Drosophila S2 cells [Exosomes]      More >>>	22437826
Drosophila wing imaginal Disc [Extracellular vesicles]      More >>>	25471885
Hematocytes [Microvesicles]      More >>>	21901833
Experiment description of studies that identified Rala in extracellular vesicles
1 Experiment ID 471 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 23035643    Organism Drosophila melanogaster Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes. Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP. Journal name Traffic Publication year 2012 Sample Drosophila S2 cells Sample name Normal-Drosophila S2 tub-Wg Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130023: EV-METRIC:33%, 56%
2 Experiment ID 1278 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 22437826    Organism Drosophila melanogaster Experiment description Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. Authors Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y, Leszyk J, Zhang B, Budnik V. Journal name J Biol Chem Publication year 2012 Sample Drosophila S2 cells Sample name S2 cells Isolation/purification methods Ultracentrifugation Density gradient centrifugation Flotation density 1.15-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120033: EV-METRIC:33%
3 Experiment ID 979 Identified molecule protein Extracellular vesicle type Extracellular vesicles Identification method Mass spectrometry PubMed ID 25471885    Organism Drosophila melanogaster Experiment description The ESCRT machinery regulates the secretion and long-range activity of Hedgehog. Authors Matusek T, Wendler F, PolèS, Pizette S, D'Angelo G, Füer M, Thénd PP. Journal name Nature Publication year 2014 Sample Drosophila wing imaginal Disc Sample name C18 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140287: EV-METRIC:11%, 22%
4 Experiment ID 161 Identified molecule protein Extracellular vesicle type Microvesicles Identification method Mass spectrometry [HCT ETD II iontrap] PubMed ID 21901833    Organism Drosophila melanogaster Experiment description Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells Authors Koppen T, Weckmann A, MüS, Staubach S, Bloch W, Dohmen RJ, Schwientek T Journal name Proteomics Publication year 2011 Sample Hematocytes Sample name Kc167 Isolation/purification methods Differential centrifugation Sucrose density gradient Ultracentrifugation Flotation density - Molecules identified in the study Protein PTM Ubiquitination Methods used in the study Western blotting Mass spectrometry [HCT ETD II iontrap] EV-TRACK EV110006: EV-METRIC:63%