Gene description for IGHA1
Gene name	immunoglobulin heavy constant alpha 1
Gene symbol	IGHA1
Other names/aliases	IgA1
Species	Homo sapiens
Database cross references - IGHA1
Vesiclepedia	VP_3493
ExoCarta	ExoCarta_3493
Entrez Gene	3493
HGNC	5478
MIM	146900
IGHA1 identified in extracellular vesicles derived from the following tissue/cell type
Ascites [Microvesicles]      More >>>	21630462
Ascites [Microvesicles]      More >>>	21630462
B cells [Exosomes]      More >>>	20458337
B cells [Exosomes]      More >>>	20458337
B cells [Exosomes]      More >>>	20458337
Breast milk [Exosomes]      More >>>	17641064
Breast milk [Extracellular vesicles]      More >>>	27601599
Breast milk [Extracellular vesicles]      More >>>	27601599
Glioblastoma cells [Microvesicles]      More >>>	19011622
Lung cancer cells [Extracellular vesicles]      More >>>	24946052
Lung cancer cells [Extracellular vesicles]      More >>>	24946052
Lung cancer cells [Extracellular vesicles]      More >>>	24946052
Mesenchymal stem cells [Exosomes]      More >>>	Unpublished / Not applicable
Saliva [Exosomes]      More >>>	19199708
Experiment description of studies that identified IGHA1 in extracellular vesicles
1 Experiment ID 158 Identified molecule protein Extracellular vesicle type Microvesicles Identification method Mass spectrometry [LTQ] PubMed ID 21630462    Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer ascites Authors Choi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS Journal name Proteomics Publication year 2011 Sample Ascites Sample name Malignant ascites - Colorectal cancer patient 2 Isolation/purification methods Differential centrifugation Sucrose density gradient OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK EV110017: EV-METRIC:38%
2 Experiment ID 159 Identified molecule protein Extracellular vesicle type Microvesicles Identification method Mass spectrometry [LTQ] PubMed ID 21630462    Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer ascites Authors Choi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS Journal name Proteomics Publication year 2011 Sample Ascites Sample name Malignant ascites - Colorectal cancer patient 3 Isolation/purification methods Differential centrifugation Sucrose density gradient OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK EV110017: EV-METRIC:38%
3 Experiment ID 79 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
4 Experiment ID 80 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
5 Experiment ID 81 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
6 Experiment ID 48 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 17641064    Organism Homo sapiens Experiment description Exosomes with immune modulatory features are present in human breast milk. Authors Admyre C, Johansson SM, Qazi KR, FiléJJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S Journal name JIMMU Publication year 2007 Sample Breast milk Sample name Breast milk - mature milk Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density 1.10-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron microscopy FACS EV-TRACK -
7 Experiment ID 574 Identified molecule protein Extracellular vesicle type Extracellular vesicles Identification method Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive] PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components Authors Van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name MCP Publication year 2016 Sample Breast milk Sample name Breast milk - normal Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
8 Experiment ID 957 Identified molecule protein Extracellular vesicle type Extracellular vesicles Identification method Mass spectrometry PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - pooled Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
9 Experiment ID 203 Identified molecule mrna Extracellular vesicle type Microvesicles Identification method Microarray PubMed ID 19011622    Organism Homo sapiens Experiment description Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers Authors Skog J, Wüer T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO Journal name Nat Cell Biol Publication year 2008 Sample Glioblastoma cells Sample name Glioblastoma cells Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Flotation density - Molecules identified in the study Protein miRNA mRNA Methods used in the study RT-PCR Antibody array Microarray EV-TRACK -
10 Experiment ID 893 Identified molecule protein Extracellular vesicle type Extracellular vesicles Identification method Mass spectrometry PubMed ID 24946052    Organism Homo sapiens Experiment description Extracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment Authors Choi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J. Journal name Proteomics Publication year 2014 Sample Lung cancer cells Sample name PC9 - gefitinib-resistant - Rep 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density Not provided Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140109: EV-METRIC:25%
11 Experiment ID 894 Identified molecule protein Extracellular vesicle type Extracellular vesicles Identification method Mass spectrometry PubMed ID 24946052    Organism Homo sapiens Experiment description Extracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment Authors Choi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J. Journal name Proteomics Publication year 2014 Sample Lung cancer cells Sample name PC9 - gefitinib-resistant - Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density Not provided Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140109: EV-METRIC:25%
12 Experiment ID 895 Identified molecule protein Extracellular vesicle type Extracellular vesicles Identification method Mass spectrometry PubMed ID 24946052    Organism Homo sapiens Experiment description Extracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment Authors Choi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J. Journal name Proteomics Publication year 2014 Sample Lung cancer cells Sample name PC9 - gefitinib-resistant - Rep 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density Not provided Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV140109: EV-METRIC:25%
13 Experiment ID 126 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antibody array Mass spectrometry EV-TRACK -
14 Experiment ID 66 Identified molecule protein Extracellular vesicle type Exosomes Identification method Mass spectrometry PubMed ID 19199708    Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron microscopy EV-TRACK -