Gene description for FASLG
Gene name Fas ligand (TNF superfamily, member 6)
Gene symbol FASLG
Other names/aliases ALPS1B
APT1LG1
CD178
CD95-L
CD95L
FASL
TNFSF6
Species Homo sapiens
 Database cross references - FASLG
Vesiclepedia VP_356
ExoCarta ExoCarta_356
Entrez Gene 356
HGNC 11936
MIM 134638
 FASLG identified in extracellular vesicles derived from the following tissue/cell type
Colorectal cancer cells [Exosomes]      More >>> 15940614   
Melanoma cells [Microvesicles]      More >>> 12021310   
Melanoma cells [Microvesicles]      More >>> 12021310   
Melanoma cells [Microvesicles]      More >>> 12021310   
Plasma [Exosomes]      More >>> 17076679   
Serum [Microvesicles]      More >>> 21606166   
T cells [Exosomes]      More >>> 21252909   
T cells [Microvesicles]      More >>> 12021310   
T cells [Microvesicles]      More >>> 10415024   
T cells [Microvesicles]      More >>> 10415024   
 Experiment description of studies that identified FASLG in extracellular vesicles
1
Experiment ID22
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 15940614   
OrganismHomo sapiens
Experiment descriptionHuman colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape.
AuthorsHuber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, Zaccheddu A, Colone M, Arancia G, Gentile M, Seregni E, Valenti R, Ballabio G, Belli F, Leo E, Parmiani G, Rivoltini L.
Journal name GASTRO
Publication year2005
SampleColorectal cancer cells
Sample nameSW403
1869col
CRC28462
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Immunoelectron microscopy
FACS
EV-TRACK -
2
Experiment ID274
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Immunoelectron microscopy
Western blotting
FACS
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleMelanoma cells
Sample nameMe15392
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImunoelectron microscopy
Western blotting
FACS
EV-TRACK -
3
Experiment ID275
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Immunoelectron microscopy
Western blotting
FACS
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleMelanoma cells
Sample name501mel
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImunoelectron microscopy
Western blotting
FACS
EV-TRACK -
4
Experiment ID276
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Immunoelectron microscopy
Western blotting
FACS
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleMelanoma cells
Sample nameMe30966
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImunoelectron microscopy
Western blotting
FACS
EV-TRACK -
5
Experiment ID43
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 17076679   
OrganismHomo sapiens
Experiment descriptionSpecific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences.
AuthorsSabapatha A, Gercel-Taylor C, Taylor DD
Journal name AJRI
Publication year2006
SamplePlasma
Sample namePlasma - Pregnant and not-pregnant women
Isolation/purification methodsSize exclusion chromatography
Ultracentrifugation
Immunobeads (CD3, CD19, CD56, CD83, PLAP)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
6
Experiment ID201
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Western blotting
PubMed ID 21606166   
OrganismHomo sapiens
Experiment descriptionBlast-derived microvesicles in sera from patients with acute myeloid leukemia suppress natural killer cell function via membrane-associated transforming growth factor-beta2
AuthorsSzczepanski MJ, Szajnik M, Welsh A, Whiteside TL, Boyiadzis M
Journal name Haematologica
Publication year2011
SampleSerum
Sample nameSerum - Acute myeloid leukemia
Isolation/purification methodsSize exclusion chromatography
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
EV-TRACK EV110116: EV-METRIC:11%
7
Experiment ID91
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 21252909   
OrganismHomo sapiens
Experiment descriptionDiacylglycerol kinase a regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes
AuthorsAlonso R, Mazzeo C, Rodriguez MC, Marsh M, Fraile-Ramos A, Calvo V, Avila-Flores A, Merida I, Izquierdo M.
Journal name CDD
Publication year2011
SampleT cells
Sample nameJ-HM1-2.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyWestern blotting
FACS
Immunofluorescent microscopy
EV-TRACK EV110031: EV-METRIC:22%
8
Experiment ID273
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Western blotting
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleT cells
Sample nameJurkat
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -
9
Experiment ID292
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Western blotting
FACS
PubMed ID 10415024   
OrganismHomo sapiens
Experiment descriptionActivated human T cells release bioactive Fas ligand and APO2 ligand in microvesicles
AuthorsMartíz-Lorenzo MJ, Anel A, Gamen S, Monle n I, Lasierra P, Larrad L, Piño A, Alava MA, Naval J
Journal name J Immunol
Publication year1999
SampleT cells
Sample nameJurkat - PHA stimulated
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
FACS
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
EV-TRACK -
10
Experiment ID293
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Western blotting
FACS
PubMed ID 10415024   
OrganismHomo sapiens
Experiment descriptionActivated human T cells release bioactive Fas ligand and APO2 ligand in microvesicles
AuthorsMartíz-Lorenzo MJ, Anel A, Gamen S, Monle n I, Lasierra P, Larrad L, Piño A, Alava MA, Naval J
Journal name J Immunol
Publication year1999
SampleT cells
Sample nameT cell blasts
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
FACS
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
FACS
EV-TRACK -