Gene description for ACTB
Gene name actin, beta
Gene symbol ACTB
Other names/aliases -
Species Ovis aries
 Database cross references - ACTB
Vesiclepedia VP_443052
ExoCarta ExoCarta_443052
Entrez Gene 443052
 ACTB identified in extracellular vesicles derived from the following tissue/cell type
T cells [Microparticles]      More >>> 16342139   
T cells [Microparticles]      More >>> 16342139   
Tracheobronchial cells [Exosomes]      More >>> 19190083   
Uterine luminal fluid [Extracellular vesicles]      More >>> 24614226   
Uterine luminal fluid [Extracellular vesicles]      More >>> 24614226   
 Experiment description of studies that identified ACTB in extracellular vesicles
1
Experiment ID306
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry [MALDI TOF/TOF]
Mass spectrometry [Q-TOF]
PubMed ID 16342139   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization
AuthorsMiguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L
Journal name Proteomics
Publication year2006
SampleT cells
Sample nameCEM T cell - PHA activation
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Mass spectrometry [QTOF]
EV-TRACK -
2
Experiment ID307
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry [MALDI TOF/TOF]
Mass spectrometry [Q-TOF]
PubMed ID 16342139   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization
AuthorsMiguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L
Journal name Proteomics
Publication year2006
SampleT cells
Sample nameCEM T cell - ActD activation
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Mass spectrometry [QTOF]
EV-TRACK -
3
Experiment ID34
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19190083   
OrganismHomo sapiens
Experiment descriptionCharacterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
AuthorsKesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name FASEB
Publication year2009
SampleTracheobronchial cells
Sample nameTracheobronchial epithelial cells
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density1.16 -1.18 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyMass spectrometry [QTOF]
Western blotting
Immunoelectron microscopy
FACS
EV-TRACK -
4
Experiment ID869
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24614226   
OrganismOvis aries
Experiment descriptionExtracellular vesicles in luminal fluid of the ovine uterus
AuthorsBurns G, Brooks K, Wildung M, Navakanitworakul R, Christenson LK, Spencer TE.
Journal name PLoS One
Publication year2014
SampleUterine luminal fluid
Sample nameUterine luminal fluid - pregnant - n=4 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140004: EV-METRIC:63%
5
Experiment ID873
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24614226   
OrganismOvis aries
Experiment descriptionExtracellular vesicles in luminal fluid of the ovine uterus
AuthorsBurns G, Brooks K, Wildung M, Navakanitworakul R, Christenson LK, Spencer TE.
Journal name PLoS One
Publication year2014
SampleUterine luminal fluid
Sample nameUterine luminal fluid - cyclic - n=4 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140004: EV-METRIC:63%