Gene description for MPP6
Gene name membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
Gene symbol MPP6
Other names/aliases PALS2
VAM-1
VAM1
p55T
Species Homo sapiens
 Database cross references - MPP6
Vesiclepedia VP_51678
ExoCarta ExoCarta_51678
Entrez Gene 51678
HGNC 18167
MIM 606959
 MPP6 identified in extracellular vesicles derived from the following tissue/cell type
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles/Ectosomes]      More >>> 24009881   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles/Ectosomes]      More >>> 24009881   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Microvesicles]      More >>> 28842968   
Colorectal cancer cells [Microvesicles]      More >>> 28842968   
Colorectal cancer cells [Extracellular vesicles]      More >>> 28842968   
Colorectal cancer cells [Extracellular vesicles]      More >>> 28842968   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Prostate cancer cells [Extracellular vesicles]      More >>> 27894104   
T cells [Microvesicles]      More >>> 28811610   
T cells [Exosomes]      More >>> 23463506   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Umblical cord mesenchymal stem cells [Microvesicles]      More >>> 29148239   
Urine [Exosomes]      More >>> 25471207   
 Experiment description of studies that identified MPP6 in extracellular vesicles
1
Experiment ID1059
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 1
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
2
Experiment ID1060
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 2
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
3
Experiment ID1061
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 3
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
4
Experiment ID1062
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373vIII - EVs 1
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -
5
Experiment ID1063
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373vIII - EVs 2
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
6
Experiment ID1064
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373vIII - EVs 3
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
7
Experiment ID79
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
8
Experiment ID80
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
9
Experiment ID81
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
10
Experiment ID583
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSF539
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
11
Experiment ID584
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSNB-19
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
12
Experiment ID586
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameU251
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
13
Experiment ID575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameBT549
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
14
Experiment ID578
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameMDA-MB-231
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
15
Experiment ID579
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameMDA-MB-468
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
16
Experiment ID574
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive]
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components
AuthorsVan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name MCP
Publication year2016
SampleBreast milk
Sample nameBreast milk - normal
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
17
Experiment ID951
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
18
Experiment ID957
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - pooled
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
19
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -
20
Experiment ID480
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDKO-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
21
Experiment ID481
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDks-8
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
22
Experiment ID482
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDLD-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%
23
Experiment ID549
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles/Ectosomes
Identification method Mass spectrometry
PubMed ID 24009881   
OrganismHomo sapiens
Experiment descriptionQuantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells.
AuthorsChoi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS.
Journal name J Extracell Vesicles
Publication year2012
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120013: EV-METRIC:50%
24
Experiment ID550
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles/Ectosomes
Identification method Mass spectrometry
PubMed ID 24009881   
OrganismHomo sapiens
Experiment descriptionQuantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells.
AuthorsChoi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS.
Journal name J Extracell Vesicles
Publication year2012
SampleColorectal cancer cells
Sample nameSW620
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120013: EV-METRIC:50%
25
Experiment ID588
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHCC 2998
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
26
Experiment ID590
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHCT-15
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
27
Experiment ID591
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHT29
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
28
Experiment ID592
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameKM12
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
29
Experiment ID696
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- insulin induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%
30
Experiment ID697
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- non-induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%
31
Experiment ID1049
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample name LIM1215 - NI
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%
32
Experiment ID1050
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample namelim1215 - II
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%
33
Experiment ID600
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameTK-10
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
34
Experiment ID601
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameUO-31
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
35
Experiment ID610
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameHOP-62
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
36
Experiment ID611
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameHOP-92
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
37
Experiment ID625
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameUACC-62
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
38
Experiment ID492
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
39
Experiment ID493
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
40
Experiment ID992
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
41
Experiment ID993
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR433
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
42
Experiment ID994
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR5
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
43
Experiment ID995
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameSKOV3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
44
Experiment ID633
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleProstate cancer cells
Sample nameDU145
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
45
Experiment ID714
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28811610   
OrganismHomo sapiens
Experiment descriptionAntibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA.
AuthorsNéth A, Orgovan N, Só BW, Osteikoetxea X, Páczi K, Szabóylor KÉ Vukman KV, Kittel Á TuriáL, Wiener Z, TóS, Drahos L, Véy K, Horvath R, BuzáEI.
Journal name Sci Rep
Publication year2017
SampleT cells
Sample nameJurkat cells - Apoptotic
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170063: EV-METRIC:75%, 75%
46
Experiment ID1005
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismHomo sapiens
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts - exosomes pulldown with EWI-2 as a bait
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130110: EV-METRIC:22%
47
Experiment ID680
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample namePreterm newborns- replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
48
Experiment ID681
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample namePreterm newborns- replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
49
Experiment ID682
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample namePreterm newborns- replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
50
Experiment ID683
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample nameTerm infants- replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
51
Experiment ID684
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample nameTerm infants- replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
52
Experiment ID685
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 29148239   
OrganismHomo sapiens
Experiment descriptionMetabolic Signature of Microvesicles from Umbilical Cord Mesenchymal Stem Cells of Preterm and Term Infants.
AuthorsBruschi M, Santucci L, Ravera S, Bartolucci M, Petretto A, Calzia D, Ghiggeri GM, Ramenghi LA, Candiano G, Panfoli I.
Journal name Proteomics Clin Appl
Publication year2018
SampleUmblical cord mesenchymal stem cells
Sample nameTerm infants- replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sonication
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
53
Experiment ID725
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 25471207   
OrganismHomo sapiens
Experiment descriptionIntraluminal proteome and peptidome of human urinary extracellular vesicles.
AuthorsLiu X, Chinello C, Musante L, Cazzaniga M, Tataruch D, Calzaferri G, James Smith A, De Sio G, Magni F, Zou H, Holthofer H.
Journal name Proteomics Clin Appl.
Publication year2015
SampleUrine
Sample nameNormal crude urine- Trypsinized
Isolation/purification methodsDifferential centrifugation
Hydrostatic filtration dialysis
Ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140189: EV-METRIC:25%