Gene description for TP53
Gene name tumor protein p53
Gene symbol TP53
Other names/aliases LFS1
P53
TRP53
Species Homo sapiens
 Database cross references - TP53
Vesiclepedia VP_7157
ExoCarta ExoCarta_7157
Entrez Gene 7157
HGNC 11998
MIM 191170
 TP53 identified in extracellular vesicles derived from the following tissue/cell type
Breast cancer cells [Microvesicles]      More >>> 25503107   
Breast cancer cells [Microvesicles]      More >>> 25503107   
Bronchial epithelial cells [Extracellular vesicles]      More >>> 29127410   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Placental mesenchymal stem cells [Exosomes]      More >>> 23861904   
Serum [Exosomes]      More >>> 22808001   
 Experiment description of studies that identified TP53 in extracellular vesicles
1
Experiment ID1230
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 25503107   
OrganismHomo sapiens
Experiment descriptionTumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN.
AuthorsMenck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, Dhople VM, Siam L, Pukrop T, Binder C, Klemm F.
Journal name J Mol Cell Biol.
Publication year2015
SampleBreast cancer cells
Sample nameMCF-7
Isolation/purification methodsUltracentrifugation
Filtration
Density gradient centrifugation
Flotation density1.13-1.22 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140270: EV-METRIC:0%, 33%, 33%
2
Experiment ID1231
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 25503107   
OrganismHomo sapiens
Experiment descriptionTumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN.
AuthorsMenck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, Dhople VM, Siam L, Pukrop T, Binder C, Klemm F.
Journal name J Mol Cell Biol.
Publication year2015
SampleBreast cancer cells
Sample nameSK-BR-3
Isolation/purification methodsUltracentrifugation
Filtration
Density gradient centrifugation
Flotation density1.13-1.22 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140270: EV-METRIC:0%, 33%, 33%
3
Experiment ID1214
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 29127410   
OrganismHomo sapiens
Experiment descriptionUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
AuthorsBenedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM.
Journal name Sci Rep.
Publication year2017
SampleBronchial epithelial cells
Sample nameUltracentrifugation, sample 3
Isolation/purification methodsUltracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170061: EV-METRIC:62%, 50%, 50%
4
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -
5
Experiment ID992
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
6
Experiment ID1135
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23861904   
OrganismHomo sapiens
Experiment descriptionExosomal signaling during hypoxia mediates microvascular endothelial cell migration and vasculogenesis.
AuthorsSalomon C, Ryan J, Sobrevia L, Kobayashi M, Ashman K, Mitchell M, Rice GE.
Journal name PLoS One
Publication year2013
SamplePlacental mesenchymal stem cells
Sample namepMSC's in 1% Oxygen
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Ultrafiltration
Density gradient centrifugation
Flotation density1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130055: EV-METRIC:33%
7
Experiment ID977
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22808001   
OrganismHomo sapiens
Experiment descriptionBiochemical and physical characterisation of urinary nanovesicles following CHAPS treatment.
AuthorsMusante L, Saraswat M, Duriez E, Byrne B, Ravidà, Domon B, Holthofer H.
Journal name PLoS One
Publication year2012
SampleSerum
Sample nameSerum -Tuberculosis patient 7
Isolation/purification methodsFiltration
ExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120006: EV-METRIC:56%