Gene description for TYK2
Gene name tyrosine kinase 2
Gene symbol TYK2
Other names/aliases JTK1
Species Homo sapiens
 Database cross references - TYK2
Vesiclepedia VP_7297
ExoCarta ExoCarta_7297
Entrez Gene 7297
HGNC 12440
MIM 176941
 TYK2 identified in extracellular vesicles derived from the following tissue/cell type
Ascites [Microvesicles]      More >>> 21630462   
Ascites [Microvesicles]      More >>> 21630462   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
Astrocytoma cells [Extracellular vesicles]      More >>> 30006486   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
B cells [Exosomes]      More >>> 20458337   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Microvesicles]      More >>> 28842968   
Colorectal cancer cells [Microvesicles]      More >>> 28842968   
Colorectal cancer cells [Extracellular vesicles]      More >>> 28842968   
Dendritic cells [Microvesicles]      More >>> 26858453   
Dendritic cells [Microvesicles]      More >>> 26858453   
Dendritic cells [Exosomes]      More >>> 26858453   
Dendritic cells [Exosomes]      More >>> 26858453   
Glioblastoma cells [Microvesicles]      More >>> 19011622   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Monocytes [Microparticles]      More >>> 19548909   
Monocytes [Microparticles]      More >>> 19548909   
Monocytes [Microparticles]      More >>> 19548909   
Monocytes [Microparticles]      More >>> 19548909   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Placental mesenchymal stem cells [Exosomes]      More >>> 23861904   
T cells [Exosomes]      More >>> 23463506   
Urine [Exosomes/Membrane vesicles]      More >>> 22106071   
 Experiment description of studies that identified TYK2 in extracellular vesicles
1
Experiment ID157
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%
2
Experiment ID159
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK EV110017: EV-METRIC:38%
3
Experiment ID1060
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 2
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
4
Experiment ID1061
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 30006486   
OrganismHomo sapiens
Experiment descriptionThe impact of oncogenic EGFRvIII on the proteome of extracellular vesicles released from glioblastoma cells.
AuthorsChoi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J.
Journal name Mol Cell Proteomics.
Publication year2018
SampleAstrocytoma cells
Sample nameU373 - EVs 3
Isolation/purification methodsFiltration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.09-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
5
Experiment ID79
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
6
Experiment ID80
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
7
Experiment ID81
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20458337   
OrganismHomo sapiens
Experiment descriptionMHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
AuthorsBuschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year2010
SampleB cells
Sample nameRN (HLA-DR15)
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [FT-ICR]
Western blotting
EV-TRACK EV100035: EV-METRIC:44%
8
Experiment ID581
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSF268
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
9
Experiment ID582
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSF295
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
10
Experiment ID584
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSNB-19
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
11
Experiment ID585
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSNB-75
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
12
Experiment ID574
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive]
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components
AuthorsVan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name MCP
Publication year2016
SampleBreast milk
Sample nameBreast milk - normal
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
13
Experiment ID951
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
14
Experiment ID956
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - D7
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV160000: EV-METRIC:50%
15
Experiment ID957
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components.
Authorsvan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name Mol Cell Proteomics
Publication year2016
SampleBreast milk
Sample nameMilk - derived EVs - pooled
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160000: EV-METRIC:50%
16
Experiment ID902
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameMEC-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
FACS
Western blotting
EV-TRACK EV150015: EV-METRIC:22%, 44%
17
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -
18
Experiment ID591
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHT29
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
19
Experiment ID592
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameKM12
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
20
Experiment ID696
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- insulin induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%
21
Experiment ID697
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- non-induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%
22
Experiment ID1049
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample name LIM1215 - NI
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%
23
Experiment ID562
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
24
Experiment ID563
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
25
Experiment ID564
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
26
Experiment ID565
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ ORBITRAP]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ ORBITRAP]
EV-TRACK -
27
Experiment ID203
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray
PubMed ID 19011622   
OrganismHomo sapiens
Experiment descriptionGlioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
AuthorsSkog J, Wüer T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO
Journal name Nat Cell Biol
Publication year2008
SampleGlioblastoma cells
Sample nameGlioblastoma cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
miRNA
mRNA
Methods used in the studyRT-PCR
Antibody array
Microarray
EV-TRACK -
28
Experiment ID598
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameRXF 393
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
29
Experiment ID605
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameMOLT-4
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
30
Experiment ID606
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameRPMI-8226
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
31
Experiment ID613
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H23
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
32
Experiment ID618
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameM14
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
33
Experiment ID619
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameMALME-3M
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
34
Experiment ID624
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameUACC-257
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
35
Experiment ID401
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameMonocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
36
Experiment ID402
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameStimulated by IgG-Monocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
37
Experiment ID403
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameStimulated by LPS-Monocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
38
Experiment ID404
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 19548909   
OrganismHomo sapiens
Experiment descriptionDifferential stimulation of monocytic cells results in distinct populations of microparticles.
AuthorsBernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD.
Journal name J Thromb Haemost
Publication year2009
SampleMonocytes
Sample nameStimulated by P-selectin-Ig-Monocytes (THP-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
39
Experiment ID492
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
40
Experiment ID493
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
41
Experiment ID627
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
42
Experiment ID628
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-4
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
43
Experiment ID1135
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23861904   
OrganismHomo sapiens
Experiment descriptionExosomal signaling during hypoxia mediates microvascular endothelial cell migration and vasculogenesis.
AuthorsSalomon C, Ryan J, Sobrevia L, Kobayashi M, Ashman K, Mitchell M, Rice GE.
Journal name PLoS One
Publication year2013
SamplePlacental mesenchymal stem cells
Sample namepMSC's in 1% Oxygen
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Ultrafiltration
Density gradient centrifugation
Flotation density1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130055: EV-METRIC:33%
44
Experiment ID1008
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismHomo sapiens
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130110: EV-METRIC:22%
45
Experiment ID437
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 22106071   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT).
AuthorsWang Z, Hill S, Luther JM, Hachey DL, Schey KL.
Journal name Proteomics
Publication year2012
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120168: EV-METRIC:14%