Gene description for Actb
Gene name actin, beta
Gene symbol Actb
Other names/aliases Actx
Species Rattus norvegicus
 Database cross references - Actb
Vesiclepedia VP_81822
ExoCarta ExoCarta_81822
Entrez Gene 81822
 Actb identified in extracellular vesicles derived from the following tissue/cell type
Hepatocytes [Exosomes]      More >>> 19367702   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Plasma [Microparticles]      More >>> 22014850   
Plasma [Microparticles]      More >>> 22014850   
Primary microglial cells [Exosomes]      More >>> 23173708   
Primary microglial cells [Exosomes]      More >>> 23173708   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
T cells [Microparticles]      More >>> 16342139   
T cells [Microparticles]      More >>> 16342139   
Tracheobronchial cells [Exosomes]      More >>> 19190083   
Urine [Exosomes]      More >>> 20535238   
 Experiment description of studies that identified Actb in extracellular vesicles
1
Experiment ID35
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19367702   
OrganismRattus norvegicus
Experiment descriptionCharacterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year2008
SampleHepatocytes
Sample nameHepatocytes
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
2
Experiment ID1279
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
3
Experiment ID1280
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
4
Experiment ID1281
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
5
Experiment ID1282
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
6
Experiment ID1283
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
7
Experiment ID1284
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
8
Experiment ID433
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22014850   
OrganismRattus norvegicus
Experiment descriptionProteomics of microparticles after experimental pulmonary embolism.
AuthorsWatts JA, Lee YY, Gellar MA, Fulkerson MB, Hwang SI, Kline JA.
Journal name Thromb Res
Publication year2012
SamplePlasma
Sample nameNormal rat-Plasma
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
9
Experiment ID434
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22014850   
OrganismRattus norvegicus
Experiment descriptionProteomics of microparticles after experimental pulmonary embolism.
AuthorsWatts JA, Lee YY, Gellar MA, Fulkerson MB, Hwang SI, Kline JA.
Journal name Thromb Res
Publication year2012
SamplePlasma
Sample nameRat of pulmonary embolism model-Plasma
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
10
Experiment ID1146
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (8 hour)
100,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV120065: EV-METRIC:22%, 22%
11
Experiment ID1148
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23173708   
OrganismRattus norvegicus
Experiment descriptionWnt3a induces exosome secretion from primary cultured rat microglia.
AuthorsHooper C, Sainz-Fuertes R, Lynham S, Hye A, Killick R, Warley A, Bolondi C, Pocock J, Lovestone S.
Journal name BMC Neurosci
Publication year2012
SamplePrimary microglial cells
Sample nameWnt3a stimulated (36 hour)
200,000g fraction
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV120065: EV-METRIC:22%, 22%
12
Experiment ID90
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
13
Experiment ID94
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
14
Experiment ID95
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
15
Experiment ID306
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry [MALDI TOF/TOF]
Mass spectrometry [Q-TOF]
PubMed ID 16342139   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization
AuthorsMiguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L
Journal name Proteomics
Publication year2006
SampleT cells
Sample nameCEM T cell - PHA activation
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Mass spectrometry [QTOF]
EV-TRACK -
16
Experiment ID307
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry [MALDI TOF/TOF]
Mass spectrometry [Q-TOF]
PubMed ID 16342139   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization
AuthorsMiguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L
Journal name Proteomics
Publication year2006
SampleT cells
Sample nameCEM T cell - ActD activation
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Mass spectrometry [QTOF]
EV-TRACK -
17
Experiment ID34
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19190083   
OrganismHomo sapiens
Experiment descriptionCharacterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
AuthorsKesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name FASEB
Publication year2009
SampleTracheobronchial cells
Sample nameTracheobronchial epithelial cells
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density1.16 -1.18 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyMass spectrometry [QTOF]
Western blotting
Immunoelectron microscopy
FACS
EV-TRACK -
18
Experiment ID413
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20535238   
OrganismRattus norvegicus
Experiment descriptionCandidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year2010
SampleUrine
Sample nameRats of liver injury-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV100012: EV-METRIC:33%, 33%