Gene description for CUL2
Gene name cullin 2
Gene symbol CUL2
Other names/aliases -
Species Homo sapiens
 Database cross references - CUL2
Vesiclepedia VP_8453
ExoCarta ExoCarta_8453
Entrez Gene 8453
HGNC 2552
MIM 603135
 CUL2 identified in extracellular vesicles derived from the following tissue/cell type
B cells [Exosomes/Microvesicles/Oncosomes/Microparticles]      More >>> 23818640   
B cells [Exosomes/Microvesicles/Oncosomes/Microparticles]      More >>> 23818640   
B cells [Exosomes/Microvesicles/Oncosomes/Microparticles]      More >>> 23818640   
B cells [Exosomes/Microvesicles/Oncosomes/Microparticles]      More >>> 23818640   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Chronic lymphocytic leukemia cells [Exosomes]      More >>> 26100252   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Colorectal cancer cells [Exosomes]      More >>> 22285593   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Extracellular vesicles]      More >>> 27894104   
Dendritic cells [Microvesicles]      More >>> 26858453   
Dendritic cells [Exosomes]      More >>> 26858453   
Dendritic cells [Exosomes]      More >>> 26858453   
Endothelial cells [Exosomes/Extracellular vesicles]      More >>> 24009886   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Lung cancer cells [Extracellular vesicles]      More >>> 24946052   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 24434149   
Urine [Exosomes/Membrane vesicles]      More >>> 22106071   
 Experiment description of studies that identified CUL2 in extracellular vesicles
1
Experiment ID534
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameEBV- and KSHV-infected B cells (JSC-1, BC1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%
2
Experiment ID535
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameEBV-infected B cells (#1, HLJ, IM9, CP)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%
3
Experiment ID536
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameKSHV-infected B cell (JC, BC3, BCP1, BCBL1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%
4
Experiment ID537
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Oncosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23818640   
OrganismHomo sapiens
Experiment descriptionModulation of B-cell exosome proteins by gamma herpesvirus infection.
AuthorsMeckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N.
Journal name Proc Natl Acad Sci U S A
Publication year2013
SampleB cells
Sample nameNormal B cells (BJAB)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130064: EV-METRIC:25%
5
Experiment ID582
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSF295
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
6
Experiment ID583
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSF539
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
7
Experiment ID586
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameU251
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
8
Experiment ID575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameBT549
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
9
Experiment ID577
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameMCF7
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
10
Experiment ID902
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 26100252   
OrganismHomo sapiens
Experiment descriptionExosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts
AuthorsPaggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim YJ, Adam J, Lichter P, Solary E, Berchem G, Moussay E.
Journal name Blood
Publication year2015
SampleChronic lymphocytic leukemia cells
Sample nameMEC-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.15-1.17 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
FACS
Western blotting
EV-TRACK EV150015: EV-METRIC:22%, 44%
11
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -
12
Experiment ID347
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 22285593   
OrganismHomo sapiens
Experiment descriptionComparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.
AuthorsTauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal name Methods
Publication year2012
SampleColorectal cancer cells
Sample nameLIM1863 - Ultracentrifugation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV120024: EV-METRIC:25%, 33%, 50%
13
Experiment ID487
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameEpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130020: EV-METRIC:38%
14
Experiment ID488
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameShed vesicles-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130020: EV-METRIC:38%
15
Experiment ID590
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHCT-15
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
16
Experiment ID592
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameKM12
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
17
Experiment ID563
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
18
Experiment ID564
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
19
Experiment ID565
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [LTQ ORBITRAP]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ ORBITRAP]
EV-TRACK -
20
Experiment ID551
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
Identification method Mass spectrometry
PubMed ID 24009886   
OrganismHomo sapiens
Experiment descriptionCellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes.
Authorsde Jong OG, Verhaar MC, Chen Y, Vader P, Gremmels H, Posthuma G, Schiffelers RM, Gucek M, van Balkom BW.
Journal name J Extracell Vesicles
Publication year2012
SampleEndothelial cells
Sample nameStressed (hypoxia, TNF-alpha-induced activation, high glucose and mannose concentrations)-Endothelial cells (HMEC-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120004: EV-METRIC:67%
21
Experiment ID598
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameRXF 393
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
22
Experiment ID599
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameSN12C
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
23
Experiment ID602
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameCCRF-CEM
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
24
Experiment ID607
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameSR
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
25
Experiment ID613
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H23
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
26
Experiment ID615
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameNCI-H460
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
27
Experiment ID890
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
28
Experiment ID891
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
29
Experiment ID893
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - gefitinib-resistant - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
30
Experiment ID894
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - gefitinib-resistant - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
31
Experiment ID895
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry
PubMed ID 24946052   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles shed from gefitinib-resistant nonsmall cell lung cancer regulate the tumor microenvironment
AuthorsChoi DY, You S, Jung JH, Lee JC, Rho JK, Lee KY, Freeman MR, Kim KP, Kim J.
Journal name Proteomics
Publication year2014
SampleLung cancer cells
Sample namePC9 - gefitinib-resistant - Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation densityNot provided
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140109: EV-METRIC:25%
32
Experiment ID619
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameMALME-3M
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
33
Experiment ID622
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
34
Experiment ID492
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
35
Experiment ID493
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130002: EV-METRIC:67%, 56%
36
Experiment ID626
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
37
Experiment ID627
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
38
Experiment ID629
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-5
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -
39
Experiment ID992
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24434149   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
AuthorsSinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T.
Journal name Biochem Biophys Res Commun.
Publication year2014
SampleOvarian cancer cells
Sample nameOVCAR3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140099: EV-METRIC:44%
40
Experiment ID437
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 22106071   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT).
AuthorsWang Z, Hill S, Luther JM, Hachey DL, Schey KL.
Journal name Proteomics
Publication year2012
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120168: EV-METRIC:14%