Gene description for HSPA4
Gene name	heat shock protein family A (Hsp70) member 4
Gene symbol	HSPA4
Other names/aliases	APG-2 HEL-S-5a HS24/P52 HSPH2 RY hsp70 hsp70RY
Species	Homo sapiens
Experiment description of studies that identified HSPA4 in Ascites
1 Experiment ID 108 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 21601258    Organism Homo sapiens Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marmé, Sün H, Altevogt P. Journal name GO Publication year 2011 Sample Ascites Sample name Malignant ascites - ovarian cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (EpCAM, CD24) Flotation density 1.04-1.15 g/mL Molecules identified in the study Protein miRNA Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK EV110020: EV-METRIC:13%, 63%, 50%
2 Experiment ID 110 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 21601258    Organism Homo sapiens Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marmé, Sün H, Altevogt P. Journal name GO Publication year 2011 Sample Ascites Sample name Ascites - liver cirrhosis Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.04-1.10 g/mL Molecules identified in the study Protein miRNA Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK EV110020: EV-METRIC:13%, 63%, 50%