Gene description for HSPA8
Gene name heat shock protein family A (Hsp70) member 8
Gene symbol HSPA8
Other names/aliases HEL-33
HEL-S-72p
HSC54
HSC70
HSC71
HSP71
HSP73
HSPA10
LAP-1
LAP1
NIP71
Species Homo sapiens
 Experiment description of studies that identified HSPA8 in Keratinocytes

1
Experiment ID32
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 18452139   
OrganismHomo sapiens
Experiment descriptionPrimary human keratinocytes externalize stratifin protein via exosomes.
AuthorsChavez-MuñC, Morse J, Kilani R, Ghahary A
Journal name JCB
Publication year2008
SampleKeratinocytes
Sample nameKeratinocytes
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Diafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID398
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19530224   
OrganismHomo sapiens
Experiment descriptionProfile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
AuthorsChavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year2009
SampleKeratinocytes
Sample nameDifferentiated-Keratinocytes
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

3
Experiment ID399
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19530224   
OrganismHomo sapiens
Experiment descriptionProfile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
AuthorsChavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year2009
SampleKeratinocytes
Sample nameUndifferentiated-Keratinocytes
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

4
Experiment ID1159
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 20409149   
OrganismHomo sapiens
Experiment descriptionTransdifferentiated circulating monocytes release exosomes containing 14-3-3 proteins with matrix metalloproteinase-1 stimulating effect for dermal fibroblasts.
AuthorsMedina A, Ghahary A.
Journal name Wound Repair Regen
Publication year2010
SampleKeratinocytes
Sample nameKLC's
Isolation/purification methodsDifferential centrifugation
Filtration
Ultrafiltration
Ultracentrifugation
Sucrose density gradient
Flotation density1.13-1.19 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV100045: EV-METRIC:25%

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