Gene description for PFN1
Gene name	profilin 1
Gene symbol	PFN1
Other names/aliases	ALS18
Species	Homo sapiens
Experiment description of studies that identified PFN1 in Glioblastoma cells
1 Experiment ID 203 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray PubMed ID 19011622    Organism Homo sapiens Experiment description Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers Authors Skog J, Wüer T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO Journal name Nat Cell Biol Publication year 2008 Sample Glioblastoma cells Sample name Glioblastoma cells Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Flotation density - Molecules identified in the study Protein miRNA mRNA Methods used in the study RT-PCR Antibody array Microarray EV-TRACK -
2 Experiment ID 722 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25802036    Organism Homo sapiens Experiment description Glioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells. Authors de Vrij J, Maas SL, Kwappenberg KM, Schnoor R, Kleijn A, Dekker L, Luider TM, de Witte LD, Litjens M, van Strien ME, Hol EM, Kroonen J, Robe PA, Lamfers ML, Schilham MW, Broekman ML. Journal name Int J Cancer Publication year 2015 Sample Glioblastoma cells Sample name U87.MG/EGFRvIII Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV150064: EV-METRIC:0%, 0%, 29%
3 Experiment ID 723 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25802036    Organism Homo sapiens Experiment description Glioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells. Authors de Vrij J, Maas SL, Kwappenberg KM, Schnoor R, Kleijn A, Dekker L, Luider TM, de Witte LD, Litjens M, van Strien ME, Hol EM, Kroonen J, Robe PA, Lamfers ML, Schilham MW, Broekman ML. Journal name Int J Cancer Publication year 2015 Sample Glioblastoma cells Sample name GS184 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV150064: EV-METRIC:0%, 0%, 29%
4 Experiment ID 1072 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 29937354    Organism Homo sapiens Experiment description Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors. Authors Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I. Journal name Cancer Cell Publication year 2018 Sample Glioblastoma cells Sample name Lethally irradiated GBM157 - apoExo - biorep2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
5 Experiment ID 1274 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23807490    Organism Homo sapiens Experiment description Glioma microvesicles carry selectively packaged coding and non-coding RNAs which alter gene expression in recipient cells. Authors Li CC, Eaton SA, Young PE, Lee M, Shuttleworth R, Humphreys DT, Grau GE, Combes V, Bebawy M, Gong J, Brammah S, Buckland ME, Suter CM. Journal name RNA Biol. Publication year 2013 Sample Glioblastoma cells Sample name U251 cells Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry Microarray EV-TRACK EV130098: EV-METRIC:29%