Gene description for ACTN4
Gene name	actinin alpha 4
Gene symbol	ACTN4
Other names/aliases	ACTININ-4 FSGS FSGS1
Species	Homo sapiens
Experiment description of studies that identified ACTN4 in Glioblastoma cells
1 Experiment ID 203 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray PubMed ID 19011622    Organism Homo sapiens Experiment description Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers Authors Skog J, Wüer T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO Journal name Nat Cell Biol Publication year 2008 Sample Glioblastoma cells Sample name Glioblastoma cells Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Flotation density - Molecules identified in the study Protein miRNA mRNA Methods used in the study RT-PCR Antibody array Microarray EV-TRACK -
2 Experiment ID 1039 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27770278    Organism Homo sapiens Experiment description Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Authors Mallawaaratchy DM, Hallal S, Russell B, Ly L, Ebrahimkhani S, Wei H, Christopherson RI, Buckland ME, Kaufman KL. Journal name J Neurooncol Publication year 2017 Sample Glioblastoma cells Sample name A172 Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.09.1.11 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
3 Experiment ID 1040 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27770278    Organism Homo sapiens Experiment description Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Authors Mallawaaratchy DM, Hallal S, Russell B, Ly L, Ebrahimkhani S, Wei H, Christopherson RI, Buckland ME, Kaufman KL. Journal name J Neurooncol Publication year 2017 Sample Glioblastoma cells Sample name LN229 Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.09.1.11 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
4 Experiment ID 1041 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27770278    Organism Homo sapiens Experiment description Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Authors Mallawaaratchy DM, Hallal S, Russell B, Ly L, Ebrahimkhani S, Wei H, Christopherson RI, Buckland ME, Kaufman KL. Journal name J Neurooncol Publication year 2017 Sample Glioblastoma cells Sample name U87MG Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.09.1.11 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK -
5 Experiment ID 1042 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27770278    Organism Homo sapiens Experiment description Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Authors Mallawaaratchy DM, Hallal S, Russell B, Ly L, Ebrahimkhani S, Wei H, Christopherson RI, Buckland ME, Kaufman KL. Journal name J Neurooncol Publication year 2017 Sample Glioblastoma cells Sample name U251 Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.09.1.11 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
6 Experiment ID 1043 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27770278    Organism Homo sapiens Experiment description Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Authors Mallawaaratchy DM, Hallal S, Russell B, Ly L, Ebrahimkhani S, Wei H, Christopherson RI, Buckland ME, Kaufman KL. Journal name J Neurooncol Publication year 2017 Sample Glioblastoma cells Sample name T98G Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.09.1.11 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
7 Experiment ID 1044 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27770278    Organism Homo sapiens Experiment description Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Authors Mallawaaratchy DM, Hallal S, Russell B, Ly L, Ebrahimkhani S, Wei H, Christopherson RI, Buckland ME, Kaufman KL. Journal name J Neurooncol Publication year 2017 Sample Glioblastoma cells Sample name CCF-STTG1 Isolation/purification methods Differential centrifugation Ultracentrifugation Filtration Density gradient centrifugation Flotation density 1.09.1.11 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
8 Experiment ID 1274 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23807490    Organism Homo sapiens Experiment description Glioma microvesicles carry selectively packaged coding and non-coding RNAs which alter gene expression in recipient cells. Authors Li CC, Eaton SA, Young PE, Lee M, Shuttleworth R, Humphreys DT, Grau GE, Combes V, Bebawy M, Gong J, Brammah S, Buckland ME, Suter CM. Journal name RNA Biol. Publication year 2013 Sample Glioblastoma cells Sample name U251 cells Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry Microarray EV-TRACK EV130098: EV-METRIC:29%