Gene description for CD81
Gene name CD81 molecule
Gene symbol CD81
Other names/aliases CVID6
S5.7
TAPA1
TSPAN28
Species Homo sapiens
 Experiment description of studies that identified CD81 in Urine

1
Experiment ID13
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 15326289   
OrganismHomo sapiens
Experiment descriptionIdentification and proteomic profiling of exosomes in human urine.
AuthorsPisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year2004
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsUltracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA XP]
Western blotting
EV-TRACK -

2
Experiment ID63
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19056867   
OrganismHomo sapiens
Experiment descriptionLarge-scale proteomics and phosphoproteomics of urinary exosomes.
AuthorsGonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year2009
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
Western blotting
EV-TRACK -

3
Experiment ID77
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method FACS
PubMed ID 20224111   
OrganismHomo sapiens
Experiment descriptionProteomics analysis of bladder cancer exosomes.
AuthorsWelton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year2010
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK EV100014: EV-METRIC:38%, 14%, 44%

4
Experiment ID78
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method FACS
PubMed ID 20224111   
OrganismHomo sapiens
Experiment descriptionProteomics analysis of bladder cancer exosomes.
AuthorsWelton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year2010
SampleUrine
Sample nameUrine - Prostate cancer
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK EV100014: EV-METRIC:38%, 14%, 44%

5
Experiment ID382
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18373357   
OrganismHomo sapiens
Experiment descriptionIsolation and identification of potential urinary microparticle biomarkers of bladder cancer.
AuthorsSmalley DM, Sheman NE, Nelson K, Theodorescu D.
Journal name J Proteome Res
Publication year2008
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID383
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18373357   
OrganismHomo sapiens
Experiment descriptionIsolation and identification of potential urinary microparticle biomarkers of bladder cancer.
AuthorsSmalley DM, Sheman NE, Nelson K, Theodorescu D.
Journal name J Proteome Res
Publication year2008
SampleUrine
Sample namePatients of bladder cancer-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

7
Experiment ID390
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane particles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19158352   
OrganismHomo sapiens
Experiment descriptionCharacterization of PKD protein-positive exosome-like vesicles.
AuthorsHogan MC, Manganelli L, Woollard JR, Masyuk AI, Masyuk TV, Tammachote R, Huang BQ, Leontovich AA, Beito TG, Madden BJ, Charlesworth MC, Torres VE, LaRusso NF, Harris PC, Ward CJ.
Journal name J Am Soc Nephrol
Publication year2009
SampleUrine
Sample namePatients of autosomal recessive polycystic kidney disease-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

8
Experiment ID437
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22106071   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT).
AuthorsWang Z, Hill S, Luther JM, Hachey DL, Schey KL.
Journal name Proteomics
Publication year2012
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120168: EV-METRIC:14%

9
Experiment ID478
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23082778   
OrganismHomo sapiens
Experiment descriptionComparative and Targeted Proteomic Analyses of Urinary Microparticles from Bladder Cancer and Hernia Patients.
AuthorsChen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT.
Journal name J Proteome Res
Publication year2012
SampleUrine
Sample namePatients of bladder cancer-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120030: EV-METRIC:33%

10
Experiment ID479
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23082778   
OrganismHomo sapiens
Experiment descriptionComparative and Targeted Proteomic Analyses of Urinary Microparticles from Bladder Cancer and Hernia Patients.
AuthorsChen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT.
Journal name J Proteome Res
Publication year2012
SampleUrine
Sample namePatients of hernia-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120030: EV-METRIC:33%

11
Experiment ID498
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23376485   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of podocyte exosome-enriched fraction from normal human urine.
AuthorsPrunotto M, Farina A, Lane L, Pernin A, Schifferli J, Hochstrasser DF, Lescuyer P, Moll S.
Journal name J Proteomics
Publication year2013
SampleUrine
Sample nameNormal-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130051: EV-METRIC:38%

12
Experiment ID512
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23533145   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
AuthorsPrincipe S, Jones EE, Kim Y, Sinha A, Nyalwidhe JO, Brooks J, Semmes OJ, Troyer DA, Lance RS, Kislinger T, Drake RR.
Journal name Proteomics
Publication year2013
SampleUrine
Sample nameLow-grade prostate cancer patient-Expressed prostatic secretions in urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130224: EV-METRIC:14%

13
Experiment ID513
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23533145   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
AuthorsPrincipe S, Jones EE, Kim Y, Sinha A, Nyalwidhe JO, Brooks J, Semmes OJ, Troyer DA, Lance RS, Kislinger T, Drake RR.
Journal name Proteomics
Publication year2013
SampleUrine
Sample nameNormal-Expressed prostatic secretions in urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130224: EV-METRIC:14%

14
Experiment ID1187
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ELISA
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa P200
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

15
Experiment ID1188
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ELISA
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa SN200
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

16
Experiment ID1186
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ELISA
Western blotting
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa P40
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

17
Experiment ID725
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 25471207   
OrganismHomo sapiens
Experiment descriptionIntraluminal proteome and peptidome of human urinary extracellular vesicles.
AuthorsLiu X, Chinello C, Musante L, Cazzaniga M, Tataruch D, Calzaferri G, James Smith A, De Sio G, Magni F, Zou H, Holthofer H.
Journal name Proteomics Clin Appl.
Publication year2015
SampleUrine
Sample nameNormal crude urine- Trypsinized
Isolation/purification methodsDifferential centrifugation
Hydrostatic filtration dialysis
Ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140189: EV-METRIC:25%

18
Experiment ID751
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29988836   
OrganismHomo sapiens
Experiment descriptionUltrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
AuthorsOeyen E, Van Mol K, Baggerman G, Willems H, Boonen K, Rolfo C, Pauwels P, Jacobs A, Schildermans K, Cho WC, Mertens I.
Journal name J Extracell Vesicles
Publication year2018
SampleUrine
Sample nameUrine - Fraction 8 and 9 pooled
Isolation/purification methodsFiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170025: EV-METRIC:62%

19
Experiment ID959
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 26196085   
OrganismHomo sapiens
Experiment descriptionIdentification of prostate cancer biomarkers in urinary exosomes.
Authors"Overbye A, Skotland T, Koehler CJ, Thiede B, Seierstad T, Berge V, Sandvig K, Llorente A.
Journal name Oncotarget
Publication year2015
SampleUrine
Sample nameUrine - prostate cancer patients
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150029: EV-METRIC:33%

20
Experiment ID958
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
Western blotting
PubMed ID 26196085   
OrganismHomo sapiens
Experiment descriptionIdentification of prostate cancer biomarkers in urinary exosomes.
Authors"Overbye A, Skotland T, Koehler CJ, Thiede B, Seierstad T, Berge V, Sandvig K, Llorente A.
Journal name Oncotarget
Publication year2015
SampleUrine
Sample nameUrine - prostate cancer control
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150029: EV-METRIC:33%

21
Experiment ID991
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 25138791   
OrganismHomo sapiens
Experiment descriptionThe exocyst and regulatory GTPases in urinary exosomes.
AuthorsChacon-Heszele MF, Choi SY, Zuo X, Baek JI, Ward C, Lipschutz JH.
Journal name Physiol Rep.
Publication year2014
SampleUrine
Sample nameUrine - normal volunteer
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140141: EV-METRIC:29%

22
Experiment ID1189
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

23
Experiment ID2754
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name10 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

24
Experiment ID2755
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name20 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

25
Experiment ID2756
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name30 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

26
Experiment ID2757
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name40 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

27
Experiment ID2758
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name50 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

28
Experiment ID2759
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsTangential flow filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

29
Experiment ID2760
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name10 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

30
Experiment ID2761
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name20 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

31
Experiment ID2762
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name30 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

32
Experiment ID2763
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name40 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

33
Experiment ID2764
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name50 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

34
Experiment ID2765
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsTangential flow filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

35
Experiment ID2766
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

36
Experiment ID2767
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

37
Experiment ID2768
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Polyethylene glycol-based precipitation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

38
Experiment ID2770
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

39
Experiment ID2771
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Phosphatidylserine affinity purification
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

40
Experiment ID2775
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameFresh
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

41
Experiment ID2776
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameStored at 4°C for 2 days
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

42
Experiment ID2777
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameStored at -80°C
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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