Gene description for Gapdh
Gene name glyceraldehyde-3-phosphate dehydrogenase
Gene symbol Gapdh
Other names/aliases Gapd
Species Rattus norvegicus
 Database cross references - Gapdh
Vesiclepedia VP_24383
ExoCarta ExoCarta_24383
Entrez Gene 24383
 Gapdh identified in extracellular vesicles derived from the following tissue/cell type
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cardiomyocytes [Exosomes]      More >>> 23376832   
Cortical neurones [Exosomes]      More >>> 16446100   
Hepatocytes [Exosomes]      More >>> 19367702   
Insulinoma cells [Exosomes]      More >>> 27872147   
Insulinoma cells [Exosomes]      More >>> 27872147   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Microparticles]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Insulinoma cells [Exosomes]      More >>> 22345510   
Pancreatic adenocarcinoma cells [Exosomes]      More >>> 20124479   
Plasma [Microparticles]      More >>> 22014850   
Plasma [Microparticles]      More >>> 22014850   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Reticulocytes [Exosomes]      More >>> 21828046   
Retinal glial cells [Exosomes]      More >>> 24400796   
T cells [Exosomes]      More >>> 23463506   
Urine [Exosomes]      More >>> 20535238   
 Experiment description of studies that identified Gapdh in extracellular vesicles
1
Experiment ID831
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 1
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
2
Experiment ID811
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 4°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
3
Experiment ID812
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 25°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
4
Experiment ID813
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 37°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
5
Experiment ID814
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes incubated at 42°C
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
6
Experiment ID832
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 2
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
7
Experiment ID833
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 3
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
8
Experiment ID835
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - mild hypoxia - Rep 1
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
9
Experiment ID836
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - mild hypoxia - Rep 2
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
10
Experiment ID837
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Western blotting
Mass spectrometry
PubMed ID 23376832   
OrganismRattus norvegicus
Experiment descriptionCardiac myocyte exosomes: stability, HSP60, and proteomics
AuthorsMalik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA.
Journal name Am J Physiol Heart Circ Physiol
Publication year2013
SampleCardiomyocytes
Sample nameCardiomyocyte cells - mild hypoxia - Rep 3
Isolation/purification methodsDifferential centrifugation
Filtration
ExoQuick
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130100: EV-METRIC:38%, 13%
11
Experiment ID30
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 16446100   
OrganismRattus norvegicus
Experiment descriptionExosomes are released by cultured cortical neurones.
AuthorsFauré, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year2006
SampleCortical neurones
Sample nameNeurones
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
12
Experiment ID35
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 19367702   
OrganismRattus norvegicus
Experiment descriptionCharacterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year2008
SampleHepatocytes
Sample nameHepatocytes
Isolation/purification methodsDifferential centrifugation
Filtration
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [QTOF]
Western blotting
EV-TRACK -
13
Experiment ID1220
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleInsulinoma cells
Sample nameINS-1E cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
14
Experiment ID1221
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 27872147   
OrganismRattus norvegicus
Experiment descriptionPrimary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity.
AuthorsCianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S.
Journal name Diabetes.
Publication year2017
SampleInsulinoma cells
Sample nameRat interleukin (IL)-1. (10 units/mL)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
15
Experiment ID1279
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
16
Experiment ID1280
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
17
Experiment ID1281
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
18
Experiment ID1282
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
19
Experiment ID1283
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
20
Experiment ID1284
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%
21
Experiment ID92
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479   
OrganismRattus norvegicus
Experiment descriptionCell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
AuthorsNazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zör M.
Journal name CR
Publication year2010
SamplePancreatic adenocarcinoma cells
Sample nameAS
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density1.14-1.17 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
EV-TRACK EV100025: EV-METRIC:44%, 44%
22
Experiment ID433
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22014850   
OrganismRattus norvegicus
Experiment descriptionProteomics of microparticles after experimental pulmonary embolism.
AuthorsWatts JA, Lee YY, Gellar MA, Fulkerson MB, Hwang SI, Kline JA.
Journal name Thromb Res
Publication year2012
SamplePlasma
Sample nameNormal rat-Plasma
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
23
Experiment ID434
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry
PubMed ID 22014850   
OrganismRattus norvegicus
Experiment descriptionProteomics of microparticles after experimental pulmonary embolism.
AuthorsWatts JA, Lee YY, Gellar MA, Fulkerson MB, Hwang SI, Kline JA.
Journal name Thromb Res
Publication year2012
SamplePlasma
Sample nameRat of pulmonary embolism model-Plasma
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -
24
Experiment ID90
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
25
Experiment ID94
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
26
Experiment ID95
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
27
Experiment ID96
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
28
Experiment ID97
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
29
Experiment ID98
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
30
Experiment ID99
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D2 Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
31
Experiment ID100
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D4 Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.16-1.21 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
32
Experiment ID101
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046   
OrganismRattus norvegicus
Experiment descriptionThe proteo-lipidic composition of exosomes changes during reticulocyte maturation.
AuthorsCarayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salomé, Joly E.
Journal name JBC
Publication year2011
SampleReticulocytes
Sample nameReticulocytes - D7 Rep 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.13-1.25 g/mL
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
EV-TRACK EV110005: EV-METRIC:44%
33
Experiment ID1259
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 24400796   
OrganismRattus norvegicus
Experiment descriptionExosomal proteins in the aqueous humor as novel biomarkers in patients with neovascular age-related macular degeneration.
AuthorsKang GY, Bang JY, Choi AJ, Yoon J, Lee WC, Choi S, Yoon S, Kim HC, Baek JH, Park HS, Lim HJ, Chung H.
Journal name J Proteome Res.
Publication year2014
SampleRetinal glial cells
Sample nameMücells
Isolation/purification methodsExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140174: EV-METRIC:13%, 13%
34
Experiment ID1007
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23463506   
OrganismMus musculus
Experiment descriptionThe intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes.
AuthorsPerez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó
Journal name J Biol Chem.
Publication year2013
SampleT cells
Sample nameT lymphoblasts - CD81 KO mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV130110: EV-METRIC:22%
35
Experiment ID413
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20535238   
OrganismRattus norvegicus
Experiment descriptionCandidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
AuthorsConde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year2010
SampleUrine
Sample nameRats of liver injury-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV100012: EV-METRIC:33%, 33%