Gene description for KRAS
Gene name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene symbol KRAS
Other names/aliases C-K-RAS
K-RAS2A
K-RAS2B
K-RAS4A
K-RAS4B
KI-RAS
KRAS1
KRAS2
NS
NS3
RASK2
Species Homo sapiens
 Database cross references - KRAS
Vesiclepedia VP_3845
ExoCarta ExoCarta_3845
Entrez Gene 3845
HGNC 6407
MIM 190070
 KRAS identified in extracellular vesicles derived from the following tissue/cell type
Ascites [Microvesicles]      More >>> 21630462   
Ascites [Microvesicles]      More >>> 21630462   
Ascites [Microvesicles]      More >>> 21630462   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Brain cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast cancer cells [Extracellular vesicles]      More >>> 27894104   
Breast milk [Extracellular vesicles]      More >>> 27601599   
Colon cancer cells [Extracellular vesicles]      More >>> 27894104   
Colon cancer cells [Extracellular vesicles]      More >>> 27894104   
Colorectal cancer cells [Exosomes]      More >>> 19837982   
Colorectal cancer cells [Microvesicles]      More >>> 19930720   
Colorectal cancer cells [Exosomes]      More >>> 22285593   
Colorectal cancer cells [Exosomes]      More >>> 22285593   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes]      More >>> 23161513   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Extracellular vesicles/Microvesicles]      More >>> 23230278   
Colorectal cancer cells [Exosomes/Membrane vesicles]      More >>> 23585443   
Colorectal cancer cells [Exosomes/Membrane vesicles]      More >>> 23585443   
Dendritic cells [Extracellular vesicle]      More >>> 26858453   
Dendritic cells [Extracellular vesicle]      More >>> 26858453   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Kidney cancer cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Leukemia cells [Extracellular vesicles]      More >>> 27894104   
Lung cancer cells [Extracellular vesicles]      More >>> 27894104   
Malignant pleural effusions [Exosomes/Microvesicles/Ectosomes/Microparticles]      More >>> 23585444   
Melanoma cells [Extracellular vesicles]      More >>> 27894104   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Exosomes]      More >>> 23333927   
Ovarian cancer cells [Extracellular vesicles]      More >>> 27894104   
Seminal fluid [Exosomes/Prostasomes]      More >>> 18819103   
Squamous carcinoma cells [Exosomes]      More >>> 20124223   
T cells [Microparticles]      More >>> 16342139   
Thymus [Exosomes]      More >>> 23844026   
Urine [Exosomes]      More >>> 15326289   
Urine [Exosomes/Membrane particles]      More >>> 19158352   
Urine [Exosomes/Membrane vesicles]      More >>> 22106071   
Urine [Exosomes]      More >>> 22418980   
Urine [Exosomes]      More >>> 22418980   
Urine [Exosomes/Membrane vesicles]      More >>> 23376485   
Urine [Exosomes/Microvesicles]      More >>> 23886663   
 Experiment description of studies that identified KRAS in extracellular vesicles
1
Experiment ID157
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
2
Experiment ID158
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 2
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
3
Experiment ID159
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 21630462   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of microvesicles derived from human colorectal cancer ascites
AuthorsChoi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS
Journal name Proteomics
Publication year2011
SampleAscites
Sample nameMalignant ascites - Colorectal cancer patient 3
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
4
Experiment ID580
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameT-47D
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
5
Experiment ID582
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSF295
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
6
Experiment ID584
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSNB-19
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
7
Experiment ID585
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameSNB-75
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
8
Experiment ID586
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBrain cancer cells
Sample nameU251
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
9
Experiment ID575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameBT549
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
10
Experiment ID579
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameMDA-MB-468
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
11
Experiment ID574
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive]
PubMed ID 27601599   
OrganismHomo sapiens
Experiment descriptionComprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components
AuthorsVan Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH
Journal name MCP
Publication year2016
SampleBreast milk
Sample nameBreast milk - normal
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density1.12-1.18 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
12
Experiment ID587
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColon cancer cells
Sample nameColo205
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
13
Experiment ID593
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColon cancer cells
Sample nameSW620
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
14
Experiment ID21
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method
Mass spectrometry   Supporting data   MS    
PubMed ID 19837982   
OrganismHomo sapiens
Experiment descriptionProteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
AuthorsSuresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year2009
SampleColorectal cancer cells
Sample nameLIM1215
Isolation/purification methodsFiltration
Ultracentrifugation
Sucrose density gradient
Flotation density1.10-1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
15
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
Optiprep density gradient
Flotation density1.098 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
16
Experiment ID349
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 22285593   
OrganismHomo sapiens
Experiment descriptionComparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.
AuthorsTauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal name Methods
Publication year2012
SampleColorectal cancer cells
Sample nameLIM1863 - OptiPrep density gradient
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
17
Experiment ID348
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry [Orbitrap]
PubMed ID 22285593   
OrganismHomo sapiens
Experiment descriptionComparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes.
AuthorsTauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal name Methods
Publication year2012
SampleColorectal cancer cells
Sample nameLIM1863 - EpCAM immunoaffinity
Isolation/purification methodsDifferential centrifugation
Filtration
Immunobeads (EpCAM)
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [Orbitrap]
Western blotting
18
Experiment ID480
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDKO-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
19
Experiment ID481
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDks-8
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
20
Experiment ID482
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDLD-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
21
Experiment ID486
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameA33 affinity purified exosomes-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
22
Experiment ID487
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameEpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
23
Experiment ID488
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles
Identification method Mass spectrometry
PubMed ID 23230278   
OrganismHomo sapiens
Experiment descriptionTwo distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.
AuthorsTauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameShed vesicles-Colorectal cancer cells (LIM1863)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
24
Experiment ID517
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 23585443   
OrganismHomo sapiens
Experiment descriptionProteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components.
AuthorsJi H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ.
Journal name Proteomics
Publication year2013
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
25
Experiment ID518
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 23585443   
OrganismHomo sapiens
Experiment descriptionProteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components.
AuthorsJi H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ.
Journal name Proteomics
Publication year2013
SampleColorectal cancer cells
Sample nameSW620
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
26
Experiment ID568
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicle
Identification method Mass spectrometry [LTQ FUSION]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Théry C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (CD9 PD)
Isolation/purification methodsDifferential centrifugation
Ultracenrifugation
Immunobeads (CD9 PD)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ FUSION]
27
Experiment ID572
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicle
Identification method Mass spectrometry [LTQ FUSION]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Théry C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (CD81 PD)
Isolation/purification methodsDifferential centrifugation
Ultracenrifugation
Immunobeads (CD81 PD)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ FUSION]
28
Experiment ID595
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameA498
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
29
Experiment ID596
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameACHN
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
30
Experiment ID598
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleKidney cancer cells
Sample nameRXF 393
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
31
Experiment ID604
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameK562
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
32
Experiment ID606
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameRPMI-8226
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
33
Experiment ID607
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLeukemia cells
Sample nameSR
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
34
Experiment ID611
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleLung cancer cells
Sample nameHOP-92
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
35
Experiment ID519
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles/Ectosomes/Microparticles
Identification method Mass spectrometry
PubMed ID 23585444   
OrganismHomo sapiens
Experiment descriptionIdentification and characterization of proteins isolated from microvesicles derived from human lung cancer pleural effusions.
AuthorsPark JO, Choi DY, Choi DS, Kim HJ, Kang JW, Jung JH, Lee JH, Kim J, Freeman MR, Lee KY, Gho YS, Kim KP.
Journal name Proteomics
Publication year2013
SampleMalignant pleural effusions
Sample nameNon-small cell lung cancer patient-Lung cancer pleural effusion
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
36
Experiment ID624
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameUACC-257
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
37
Experiment ID492
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameIGROV1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
38
Experiment ID493
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23333927   
OrganismHomo sapiens
Experiment descriptionCharacterization and proteomic analysis of ovarian cancer-derived exosomes.
AuthorsLiang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year2013
SampleOvarian cancer cells
Sample nameOVCAR-3
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
39
Experiment ID628
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleOvarian cancer cells
Sample nameOVCAR-4
Isolation/purification methodsSerum-free medium
Differential centrifugation
polymer-based precipitation
Ultracenrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
40
Experiment ID389
Identified moleculeprotein
Extracellular vesicle typeExosomes/Prostasomes
Identification method Mass spectrometry
PubMed ID 18819103   
OrganismHomo sapiens
Experiment descriptionStructural heterogeneity and protein composition of exosome-like vesicles (prostasomes) in human semen.
AuthorsPoliakov A, Spilman M, Dokland T, Amling CL, Mobley JA.
Journal name Prostate
Publication year2009
SampleSeminal fluid
Sample nameNormal donors-Seminal fluid
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
41
Experiment ID411
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 20124223   
OrganismHomo sapiens
Experiment descriptionHypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
AuthorsPark JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year2010
SampleSquamous carcinoma cells
Sample nameSquamous carcinoma cell (A431)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
42
Experiment ID307
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
Identification method Mass spectrometry [MALDI TOF/TOF]
Mass spectrometry [Q-TOF]
PubMed ID 16342139   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization
AuthorsMiguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L
Journal name Proteomics
Publication year2006
SampleT cells
Sample nameCEM T cell - ActD activation
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [MALDI TOF/TOF]
Mass spectrometry [QTOF]
43
Experiment ID538
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 23844026   
OrganismHomo sapiens
Experiment descriptionCharacterization of human thymic exosomes.
AuthorsSkogberg G, Gudmundsdottir J, van der Post S, Sandström K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year2013
SampleThymus
Sample nameNormal-Thymus
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
44
Experiment ID13
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 15326289   
OrganismHomo sapiens
Experiment descriptionIdentification and proteomic profiling of exosomes in human urine.
AuthorsPisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year2004
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsUltracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA XP]
Western blotting
45
Experiment ID390
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane particles
Identification method Mass spectrometry
PubMed ID 19158352   
OrganismHomo sapiens
Experiment descriptionCharacterization of PKD protein-positive exosome-like vesicles.
AuthorsHogan MC, Manganelli L, Woollard JR, Masyuk AI, Masyuk TV, Tammachote R, Huang BQ, Leontovich AA, Beito TG, Madden BJ, Charlesworth MC, Torres VE, LaRusso NF, Harris PC, Ward CJ.
Journal name J Am Soc Nephrol
Publication year2009
SampleUrine
Sample namePatients of autosomal recessive polycystic kidney disease-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
46
Experiment ID437
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 22106071   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT).
AuthorsWang Z, Hill S, Luther JM, Hachey DL, Schey KL.
Journal name Proteomics
Publication year2012
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
47
Experiment ID444
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22418980   
OrganismHomo sapiens
Experiment descriptionA multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
AuthorsRaj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year2012
SampleUrine
Sample nameNormal_high density-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
48
Experiment ID445
Identified moleculeprotein
Extracellular vesicle typeExosomes
Identification method Mass spectrometry
PubMed ID 22418980   
OrganismHomo sapiens
Experiment descriptionA multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
AuthorsRaj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year2012
SampleUrine
Sample nameNormal_low density-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
49
Experiment ID498
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
Identification method Mass spectrometry
PubMed ID 23376485   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of podocyte exosome-enriched fraction from normal human urine.
AuthorsPrunotto M, Farina A, Lane L, Pernin A, Schifferli J, Hochstrasser DF, Lescuyer P, Moll S.
Journal name J Proteomics
Publication year2013
SampleUrine
Sample nameNormal-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
50
Experiment ID540
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
Identification method Mass spectrometry
PubMed ID 23886663   
OrganismHomo sapiens
Experiment descriptionLRRK2 Secretion in Exosomes is Regulated by 14-3-3.
AuthorsFraser KB, Moehle MS, Daher JP, Webber PJ, Williams JY, Stewart CA, Yacoubian TA, Cowell RM, Dokland T, Ye T, Chen D, Siegal GP, Galemmo RA, Tsika E, Moore DJ, Standaert DG, Kojima K, Mobley JA, West AB.
Journal name Hum Mol Genet
Publication year2013
SampleUrine
Sample nameNormal-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry