Gene description for ADAM10
Gene name	ADAM metallopeptidase domain 10
Gene symbol	ADAM10
Other names/aliases	AD10 AD18 CD156c CDw156 HsT18717 MADM RAK kuz
Species	Homo sapiens
Experiment description of studies that identified ADAM10 in Ascites
1 Experiment ID 108 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 21601258    Organism Homo sapiens Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marmé, Sün H, Altevogt P. Journal name GO Publication year 2011 Sample Ascites Sample name Malignant ascites - ovarian cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (EpCAM, CD24) Flotation density 1.04-1.15 g/mL Molecules identified in the study Protein miRNA Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK EV110020: EV-METRIC:13%, 63%, 50%
2 Experiment ID 110 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 21601258    Organism Homo sapiens Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marmé, Sün H, Altevogt P. Journal name GO Publication year 2011 Sample Ascites Sample name Ascites - liver cirrhosis Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.04-1.10 g/mL Molecules identified in the study Protein miRNA Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK EV110020: EV-METRIC:13%, 63%, 50%
3 Experiment ID 131 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting FACS PubMed ID 19188015    Organism Homo sapiens Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes Authors Keller S, Kö AK, Marmé, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P. Journal name CL Publication year 2009 Sample Ascites Sample name Malignant ascites - Ovarian cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.08–-1.14 g/mL Molecules identified in the study Protein Lipids Methods used in the study Cytofluorographic analysis Annexin V-FITC Western blotting FACS EV-TRACK -
4 Experiment ID 157 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 21630462    Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer ascites Authors Choi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS Journal name Proteomics Publication year 2011 Sample Ascites Sample name Malignant ascites - Colorectal cancer patient 1 Isolation/purification methods Differential centrifugation Sucrose density gradient OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK EV110017: EV-METRIC:38%