Gene description for Mfge8
Gene name	milk fat globule EGF and factor V/VIII domain containing
Gene symbol	Mfge8
Other names/aliases	AGS MFGME8 MFGMP-E8 OAcGD3S
Species	Rattus norvegicus
Experiment description of studies that identified Mfge8 in Adipocytes
1 Experiment ID 206 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 21435393    Organism Rattus norvegicus Experiment description Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis Authors MüG, Schneider M, Biemer-Daub G, Wied S. Journal name Cell Signal Publication year 2011 Sample Adipocytes Sample name Adipocytes - Large Isolation/purification methods Differential centrifugation Immunobeads (Gce1/CD73) Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK EV110050: EV-METRIC:43%, 29%
2 Experiment ID 207 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 21435393    Organism Rattus norvegicus Experiment description Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis Authors MüG, Schneider M, Biemer-Daub G, Wied S. Journal name Cell Signal Publication year 2011 Sample Adipocytes Sample name Stromal vascular cells - Differentiated adipocytes Isolation/purification methods Differential centrifugation Sucrose density gradient Size exclusion chromatography Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK EV110050: EV-METRIC:43%, 29%
3 Experiment ID 209 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 21435393    Organism Rattus norvegicus Experiment description Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis Authors MüG, Schneider M, Biemer-Daub G, Wied S. Journal name Cell Signal Publication year 2011 Sample Adipocytes Sample name Adipocytes - Small Isolation/purification methods Differential centrifugation Immunobeads (Gce1/CD73) Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK EV110050: EV-METRIC:43%, 29%
4 Experiment ID 305 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 17478559    Organism Rattus norvegicus Experiment description Identification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth 8-associated microvesicles Authors Aoki N, Jin-no S, Nakagawa Y, Asai N, Arakawa E, Tamura N, Tamura T, Matsuda T Journal name Endocrinology Publication year 2007 Sample Adipocytes Sample name Adipocytes Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 338 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 20621060    Organism Rattus norvegicus Experiment description Adipocyte-derived microvesicles contain RNA that is transported into macrophages and might be secreted into blood circulation Authors Ogawa R, Tanaka C, Sato M, Nagasaki H, Sugimura K, Okumura K, Nakagawa Y, Aoki N Journal name Biochem Biophys Res Commun Publication year 2010 Sample Adipocytes Sample name 3T3-L1 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK -