Gene description for Lep
Gene name	leptin
Gene symbol	Lep
Other names/aliases	OB obese
Species	Rattus norvegicus
Experiment description of studies that identified Lep in Adipocytes
1 Experiment ID 206 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 21435393    Organism Rattus norvegicus Experiment description Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis Authors MüG, Schneider M, Biemer-Daub G, Wied S. Journal name Cell Signal Publication year 2011 Sample Adipocytes Sample name Adipocytes - Large Isolation/purification methods Differential centrifugation Immunobeads (Gce1/CD73) Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK EV110050: EV-METRIC:43%, 29%
2 Experiment ID 207 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 21435393    Organism Rattus norvegicus Experiment description Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis Authors MüG, Schneider M, Biemer-Daub G, Wied S. Journal name Cell Signal Publication year 2011 Sample Adipocytes Sample name Stromal vascular cells - Differentiated adipocytes Isolation/purification methods Differential centrifugation Sucrose density gradient Size exclusion chromatography Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK EV110050: EV-METRIC:43%, 29%
3 Experiment ID 209 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 21435393    Organism Rattus norvegicus Experiment description Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis Authors MüG, Schneider M, Biemer-Daub G, Wied S. Journal name Cell Signal Publication year 2011 Sample Adipocytes Sample name Adipocytes - Small Isolation/purification methods Differential centrifugation Immunobeads (Gce1/CD73) Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK EV110050: EV-METRIC:43%, 29%
4 Experiment ID 338 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method RT-PCR PubMed ID 20621060    Organism Rattus norvegicus Experiment description Adipocyte-derived microvesicles contain RNA that is transported into macrophages and might be secreted into blood circulation Authors Ogawa R, Tanaka C, Sato M, Nagasaki H, Sugimura K, Okumura K, Nakagawa Y, Aoki N Journal name Biochem Biophys Res Commun Publication year 2010 Sample Adipocytes Sample name 3T3-L1 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study mRNA miRNA Methods used in the study RT-PCR EV-TRACK -