Gene description for ANXA2
Gene name annexin A2
Gene symbol ANXA2
Other names/aliases ANX2
ANX2L4
CAL1H
HEL-S-270
LIP2
LPC2
LPC2D
P36
PAP-IV
Species Homo sapiens
 Experiment description of studies that identified ANXA2 in Macrophage

1
Experiment ID671
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29953960   
OrganismHomo sapiens
Experiment descriptionExosomes derived from calcium oxalate-exposed macrophages enhance IL-8 production from renal cells, neutrophil migration and crystal invasion through extracellular matrix.
AuthorsSinghto N, Thongboonkerd V.
Journal name J Proteomics
Publication year2018
SampleMacrophage
Sample nameU937- Human monocytic cell line differentiated to macrophages (Control)
Isolation/purification methodsMicrofiltration
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

2
Experiment ID672
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29953960   
OrganismHomo sapiens
Experiment descriptionExosomes derived from calcium oxalate-exposed macrophages enhance IL-8 production from renal cells, neutrophil migration and crystal invasion through extracellular matrix.
AuthorsSinghto N, Thongboonkerd V.
Journal name J Proteomics
Publication year2018
SampleMacrophage
Sample nameU937- Human monocytic cell line differentiated to macrophages (COM treated)
Isolation/purification methodsMicrofiltration
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

3
Experiment ID1012
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles/Exosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22711894   
OrganismHomo sapiens
Experiment descriptionBiochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages.
AuthorsKadiu I, Narayanasamy P, Dash PK, Zhang W, Gendelman HE.
Journal name J Immunol.
Publication year2012
SampleMacrophage
Sample nameMonocyte-derived macrophages - uninfected
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120137: EV-METRIC:17%, 0%

4
Experiment ID1013
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22711894   
OrganismHomo sapiens
Experiment descriptionBiochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages.
AuthorsKadiu I, Narayanasamy P, Dash PK, Zhang W, Gendelman HE.
Journal name J Immunol.
Publication year2012
SampleMacrophage
Sample nameMonocyte-derived macrophages - HLA-DR pulldwon microvesicles
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry
EV-TRACK EV120137: EV-METRIC:17%, 0%

5
Experiment ID1014
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22711894   
OrganismHomo sapiens
Experiment descriptionBiochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages.
AuthorsKadiu I, Narayanasamy P, Dash PK, Zhang W, Gendelman HE.
Journal name J Immunol.
Publication year2012
SampleMacrophage
Sample nameMonocyte-derived macrophages - CHMP4/TSG101 pulldown exosomes
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry
EV-TRACK EV120137: EV-METRIC:17%, 0%

6
Experiment ID1015
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22711894   
OrganismHomo sapiens
Experiment descriptionBiochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages.
AuthorsKadiu I, Narayanasamy P, Dash PK, Zhang W, Gendelman HE.
Journal name J Immunol.
Publication year2012
SampleMacrophage
Sample nameMonocyte-derived macrophages - Myosin B pulldown exosomes
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry
EV-TRACK EV120137: EV-METRIC:17%, 0%

7
Experiment ID1016
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22711894   
OrganismHomo sapiens
Experiment descriptionBiochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages.
AuthorsKadiu I, Narayanasamy P, Dash PK, Zhang W, Gendelman HE.
Journal name J Immunol.
Publication year2012
SampleMacrophage
Sample nameMonocyte-derived macrophages - Rab 11
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry
EV-TRACK EV120137: EV-METRIC:17%, 0%

8
Experiment ID1017
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22711894   
OrganismHomo sapiens
Experiment descriptionBiochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages.
AuthorsKadiu I, Narayanasamy P, Dash PK, Zhang W, Gendelman HE.
Journal name J Immunol.
Publication year2012
SampleMacrophage
Sample nameMonocyte-derived macrophages - Vinculin/Talin pulldown exosomes
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyMass spectrometry
EV-TRACK EV120137: EV-METRIC:17%, 0%

9
Experiment ID673
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
Western blotting
PubMed ID 29953960   
OrganismHomo sapiens
Experiment descriptionExosomes derived from calcium oxalate-exposed macrophages enhance IL-8 production from renal cells, neutrophil migration and crystal invasion through extracellular matrix.
AuthorsSinghto N, Thongboonkerd V.
Journal name J Proteomics
Publication year2018
SampleMacrophage
Sample nameU937- Human monocytic cell line differentiated to macrophages (Control)
Isolation/purification methodsMicrofiltration
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

10
Experiment ID674
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
Western blotting
PubMed ID 29953960   
OrganismHomo sapiens
Experiment descriptionExosomes derived from calcium oxalate-exposed macrophages enhance IL-8 production from renal cells, neutrophil migration and crystal invasion through extracellular matrix.
AuthorsSinghto N, Thongboonkerd V.
Journal name J Proteomics
Publication year2018
SampleMacrophage
Sample nameU937- Human monocytic cell line differentiated to macrophages (COM treated)
Isolation/purification methodsMicrofiltration
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

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