Gene description for EPCAM
Gene name	epithelial cell adhesion molecule
Gene symbol	EPCAM
Other names/aliases	Ber-Ep4 BerEp4 DIAR5 EGP-2 EGP314 EGP40 ESA HNPCC8 KS1/4 KSA LYNCH8 M4S1 MIC18 MK-1 MOC-31 TACSTD1 TROP1
Species	Homo sapiens
Experiment description of studies that identified EPCAM in Ascites
1 Experiment ID 108 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 21601258    Organism Homo sapiens Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marmé, Sün H, Altevogt P. Journal name GO Publication year 2011 Sample Ascites Sample name Malignant ascites - ovarian cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (EpCAM, CD24) Flotation density 1.04-1.15 g/mL Molecules identified in the study Protein miRNA Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK EV110020: EV-METRIC:13%, 63%, 50%
2 Experiment ID 131 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 19188015    Organism Homo sapiens Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes Authors Keller S, Kö AK, Marmé, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P. Journal name CL Publication year 2009 Sample Ascites Sample name Malignant ascites - Ovarian cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.08–-1.14 g/mL Molecules identified in the study Protein Lipids Methods used in the study Cytofluorographic analysis Annexin V-FITC Western blotting FACS EV-TRACK -
3 Experiment ID 157 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 21630462    Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer ascites Authors Choi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS Journal name Proteomics Publication year 2011 Sample Ascites Sample name Malignant ascites - Colorectal cancer patient 1 Isolation/purification methods Differential centrifugation Sucrose density gradient OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK EV110017: EV-METRIC:38%
4 Experiment ID 158 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 21630462    Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer ascites Authors Choi DS, Park JO, Jang SC, Yoon YJ, Jung JW, Choi DY, Kim JW, Kang JS, Park J, Hwang D, Lee KH, Park SH, Kim YK, Desiderio DM, Kim KP, Gho YS Journal name Proteomics Publication year 2011 Sample Ascites Sample name Malignant ascites - Colorectal cancer patient 2 Isolation/purification methods Differential centrifugation Sucrose density gradient OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK EV110017: EV-METRIC:38%
5 Experiment ID 238 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Dot blot PubMed ID 21293856    Organism Homo sapiens Experiment description Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC Authors Battke C, Ruiss R, Welsch U, Wimberger P, Lang S, Jochum S, Zeidler R Journal name Cancer Immunol Immunother Publication year 2011 Sample Ascites Sample name Malignant ascites - Ovarian cancer Isolation/purification methods Differential centrifugation Filtration Ultrafiltration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.17 g/mL Molecules identified in the study Protein Methods used in the study Dot blot EV-TRACK EV110080: EV-METRIC:14%, 14%, 38%