Gene description for CD9
Gene name CD9 molecule
Gene symbol CD9
Other names/aliases BTCC-1
DRAP-27
MIC3
MRP-1
TSPAN-29
TSPAN29
Species Homo sapiens
 Experiment description of studies that identified CD9 in Saliva

1
Experiment ID66
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19199708   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
AuthorsGonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year2009
SampleSaliva
Sample nameSaliva
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
Western blotting
Immunoelectron microscopy
EV-TRACK -

2
Experiment ID122
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 21235781   
OrganismHomo sapiens
Experiment descriptionHuman saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
AuthorsLäer C, Alikhani VS, Ekströ, Eldh M, Paredes PT, Bossios A, Sjöand M, Gabrielsson S, Löll J, Valadi H.
Journal name JTM
Publication year2011
SampleSaliva
Sample nameSaliva - Normal
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Immunoelectron microscopy
Western blotting
EV-TRACK EV110029: EV-METRIC:14%, 29%, 14%

3
Experiment ID1391
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled
Isolation/purification methodsCentrifugation
Filtration
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1392
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled (13.1 µg protein)
Isolation/purification methodsDifferential centrifugation
Tangential flow filtration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID1393
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled (7.1 µg protein)
Isolation/purification methodsDifferential centrifugation
Tangential flow filtration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID2496
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34368811   
OrganismHomo sapiens
Experiment descriptionIsolation and characterization of extracellular vesicles in saliva of children with asthma
AuthorsComfort N, Bloomquist TR, Shephard AP, Petty CR, Cunningham A, Hauptman M, Phipatanakul W, Baccarelli A.
Journal name Extracell Vesicles Circ Nucl Acids
Publication year2021
SampleSaliva
Sample nameCell-free saliva - children (4 -14 year old) with asthma
Isolation/purification methodsExoQuick Kit
Centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
ExoView immunocapture
EV-TRACK -

7
Experiment ID2772
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleSaliva
Sample nameSaliva
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID2773
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleSaliva
Sample nameSaliva
Isolation/purification methodsFiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID2774
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleSaliva
Sample nameSaliva
Isolation/purification methodsFiltration
Polyethylene glycol-based precipitation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID1394
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled (CD9, CD63, and CD81-positive EVs)
Isolation/purification methodsDifferential centrifugation
Tangential flow filtration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyFlow cytometry
EV-TRACK -

11
Experiment ID1395
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled (CD9-positive EVs)
Isolation/purification methodsDifferential centrifugation
Tangential flow filtration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyFlow cytometry
EV-TRACK -

12
Experiment ID1396
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled (CD63-positive EVs)
Isolation/purification methodsDifferential centrifugation
Tangential flow filtration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyFlow cytometry
EV-TRACK -

13
Experiment ID1397
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Flow cytometry
PubMed ID 32939236   
OrganismHomo sapiens
Experiment descriptionSalivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection
AuthorsConzelmann C, Groß R, Zou M, Krüger F, Görgens A, Gustafsson MO, El Andaloussi S, Münch J, Müller JA.
Journal name J Extracell Vesicles
Publication year2020
SampleSaliva
Sample nameSaliva - pooled (CD81-positive EVs)
Isolation/purification methodsDifferential centrifugation
Tangential flow filtration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyFlow cytometry
EV-TRACK -

14
Experiment ID2496
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ExoView immunocapture
PubMed ID 34368811   
OrganismHomo sapiens
Experiment descriptionIsolation and characterization of extracellular vesicles in saliva of children with asthma
AuthorsComfort N, Bloomquist TR, Shephard AP, Petty CR, Cunningham A, Hauptman M, Phipatanakul W, Baccarelli A.
Journal name Extracell Vesicles Circ Nucl Acids
Publication year2021
SampleSaliva
Sample nameCell-free saliva - children (4 -14 year old) with asthma
Isolation/purification methodsExoQuick Kit
Centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
ExoView immunocapture
EV-TRACK -

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